8PET
Cryo-EM structure of the full-length human alpha1beta3 GABA(A) receptor (babba arrangement) in complex with nanobody Nb25, expressed with a 5-fold excess of beta3 over alpha1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, C) | Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 484 | 54100.1 | 2 | UniProt (P14867) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit alpha-1 |
| 2 | B, C, E (B, D, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 490 | 55954.3 | 3 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (human) | GABA(A) receptor subunit beta-3,GABAAR subunit beta-3 |
| 3 | F (F) | Nanobody Nb25 | polymer | 135 | 14928.3 | 1 | Lama glama | ||
| 4 | G, H, J, L, M (a, b, c, d, e) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 5 | In PDB GlyTouCan (G22768VO) | |||
| 5 | I, K, N (G, H, I) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 6 | AA, O (C, A) | HEXADECANE | non-polymer | 226.4 | 2 | Chemie (R16) | |||
| 7 | BA, P (C, A) | [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate | non-polymer | 746.6 | 2 | Chemie (PIO) | |||
| 8 | CA, DA, EA, Q, R... (E, B, D) | DECANE | non-polymer | 142.3 | 12 | Chemie (D10) | |||
| 9 | U (B) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 10 | FA, GA, HA, IA, JA... (A, B, D, C, E...) | water | water | 18.0 | 84 | Chemie (HOH) |
Sequence modifications
A, C: 10 - 429 (UniProt: P14867)
B, D, E: 1 - 473 (UniProt: P28472)
| PDB | External Database | Details |
|---|---|---|
| Met -54 | - | initiating methionine |
| Gly -53 | - | expression tag |
| Ile -52 | - | expression tag |
| Leu -51 | - | expression tag |
| Pro -50 | - | expression tag |
| Ser -49 | - | expression tag |
| Pro -48 | - | expression tag |
| Gly -47 | - | expression tag |
| Met -46 | - | expression tag |
| Pro -45 | - | expression tag |
| Ala -44 | - | expression tag |
| Leu -43 | - | expression tag |
| Leu -42 | - | expression tag |
| Ser -41 | - | expression tag |
| Leu -40 | - | expression tag |
| Val -39 | - | expression tag |
| Ser -38 | - | expression tag |
| Leu -37 | - | expression tag |
| Leu -36 | - | expression tag |
| Ser -35 | - | expression tag |
| Val -34 | - | expression tag |
| Leu -33 | - | expression tag |
| Leu -32 | - | expression tag |
| Met -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Cys -29 | - | expression tag |
| Val -28 | - | expression tag |
| Ala -27 | - | expression tag |
| Glu -26 | - | expression tag |
| Thr -25 | - | expression tag |
| Gly -24 | - | expression tag |
| Trp -23 | - | expression tag |
| Ser -22 | - | expression tag |
| His -21 | - | expression tag |
| Pro -20 | - | expression tag |
| Gln -19 | - | expression tag |
| Phe -18 | - | expression tag |
| Glu -17 | - | expression tag |
| Lys -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Gly -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Gly -10 | - | expression tag |
| Gly -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Gly -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Trp -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Pro 0 | - | expression tag |
| Gln 1 | - | expression tag |
| Phe 2 | - | expression tag |
| Glu 3 | - | expression tag |
| Lys 4 | - | expression tag |
| Gly 5 | - | expression tag |
| Gly 6 | - | expression tag |
| Ser 7 | - | expression tag |
| Thr 8 | - | expression tag |
| Gly 9 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met -39 | - | initiating methionine |
| Gly -38 | - | expression tag |
| Ile -37 | - | expression tag |
| Leu -36 | - | expression tag |
| Pro -35 | - | expression tag |
| Ser -34 | - | expression tag |
| Pro -33 | - | expression tag |
| Gly -32 | - | expression tag |
| Met -31 | - | expression tag |
| Pro -30 | - | expression tag |
| Ala -29 | - | expression tag |
| Leu -28 | - | expression tag |
| Leu -27 | - | expression tag |
| Ser -26 | - | expression tag |
| Leu -25 | - | expression tag |
| Val -24 | - | expression tag |
| Ser -23 | - | expression tag |
| Leu -22 | - | expression tag |
| Leu -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Val -19 | - | expression tag |
| Leu -18 | - | expression tag |
| Leu -17 | - | expression tag |
| Met -16 | - | expression tag |
| Gly -15 | - | expression tag |
| Cys -14 | - | expression tag |
| Val -13 | - | expression tag |
| Ala -12 | - | expression tag |
| Glu -11 | - | expression tag |
| Tyr -10 | - | expression tag |
| Pro -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Asp -7 | - | expression tag |
| Val -6 | - | expression tag |
| Pro -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Tyr -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Thr -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Gly 424 | - | insertion |
| Thr 425 | - | insertion |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 290991.4 | |
| Branched | Number of molecules | 8 |
| Total formula weight | 5827.3 | |
| Non-Polymers* | Number of molecules | 17 |
| Total formula weight | 3718.8 | |
| All* | Total formula weight | 300537.5 |
