8PB9

Cryo-EM structure of the c-di-GMP-bound FleQ-FleN master regulator complex from Pseudomonas aeruginosa

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	D, C, E	Transcriptional regulator FleQ	polymer	396	44884.3	3	UniProt (G3XCV0) Pfam (PF06490) Pfam (PF00158) In PDB	Pseudomonas aeruginosa PAO1
2	A, B	Antiactivator FleN	polymer	284	30327.1	2	UniProt (G3XD64) Pfam (PF10609) In PDB	Pseudomonas aeruginosa PAO1
3	D	9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)	non-polymer		690.4	2	Chemie (C2E)
4	A, B	PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER	non-polymer		505.2	2	Chemie (ACP)

Sequence modifications

D, C, E: 2 - 394 (UniProt: G3XCV0)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ser 1	-	expression tag

A, B: 2 - 280 (UniProt: G3XD64)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Met -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 48	Asp 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	195307.2
Non-Polymers*	Number of molecules	4
	Total formula weight	2391.2
All*	Total formula weight	197698.5

*Water molecules are not included.