8PB9
Cryo-EM structure of the c-di-GMP-bound FleQ-FleN master regulator complex from Pseudomonas aeruginosa
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D, E (D, C, E) | Transcriptional regulator FleQ | polymer | 396 | 44884.3 | 3 | UniProt (G3XCV0) Pfam (PF06490) Pfam (PF00158) | Pseudomonas aeruginosa PAO1 | |
| 2 | B, C (A, B) | Antiactivator FleN | polymer | 284 | 30327.1 | 2 | UniProt (G3XD64) Pfam (PF10609) | Pseudomonas aeruginosa PAO1 | |
| 3 | F, G (D) | 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one) | non-polymer | 690.4 | 2 | Chemie (C2E) | |||
| 4 | H, I (A, B) | PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER | non-polymer | 505.2 | 2 | Chemie (ACP) |
Sequence modifications
D, C, E: 2 - 394 (UniProt: G3XCV0)
A, B: 2 - 280 (UniProt: G3XD64)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | initiating methionine |
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Met -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| Ala 48 | Asp 48 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 195307.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 2391.2 | |
| All* | Total formula weight | 197698.5 |
