8P3H

Fusion hSlp2-a_Rab27A bound to covalent hit fragment IMP-1712

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Synaptotagmin-like protein 2,Ras-related protein Rab-27A	polymer	230	25914.2	2	UniProt (Q9HCH5) UniProt (P51159) Pfam (PF02318) Pfam (PF00071)	Homo sapiens (human)	Breast cancer-associated antigen SGA-72M,Exophilin-4,Rab-27,GTP-binding protein Ram
2	C, I (A, B)	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	2	Chemie (GNP)
3	D, E, F, J (A, B)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	G, K (A, B)	methyl (2~{S})-1-[4-(propanoylamino)phenyl]sulfonylpyrrolidine-2-carboxylate	non-polymer		340.4	2	Chemie (WTO)
5	H, L (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	M, N (A, B)	water	water		18.0	47	Chemie (HOH)

Sequence modifications

A, B: -35 - -9 (UniProt: Q9HCH5)

PDB	External Database	Details
Gly -38	-	expression tag
His -37	-	expression tag
Met -36	-	expression tag
Gly -9	-	linker
Ser -9	-	linker
Gly -9	-	linker
Ser -9	-	linker
Gly -9	-	linker
Ser -9	-	linker
Gly -9	-	linker

A, B: -9 - 174 (UniProt: P51159)

PDB	External Database	Details
Leu 60	Gln 78	engineered mutation
Ser 170	Cys 188	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51828.4
Non-Polymers*	Number of molecules	10
	Total formula weight	2142.2
All*	Total formula weight	53970.6

*Water molecules are not included.