8P3H

Fusion hSlp2-a_Rab27A bound to covalent hit fragment IMP-1712

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	60.172, 77.696, 116.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.080 - 2.760
R-factor	0.2309
Rwork	0.227
R-free	0.31280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.131
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.080	2.859
High resolution limit [Å]	2.760	2.760
Number of reflections	14556	1419
<I/σ(I)>	5.21
Completeness [%]	99.7
Redundancy	2
CC(1/2)	0.972	0.227

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M NH4SO4, 0.1 M MES, pH 6.0, 15% PEG 4K