8P1H

Crystal structure of the chimera of human 14-3-3 zeta and phosphorylated cytoplasmic loop fragment of the alpha7 acetylcholine receptor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta,Neuronal acetylcholine receptor subunit alpha-7	polymer	248	27816.3	2	UniProt (E7EX29) UniProt (P36544) Pfam (PF00244) UniProt (by SIFTS) (P63104)	Homo sapiens (human)
2	C, G, H, J (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
3	D, E, F (A)	AZIDE ION	non-polymer		42.0	3	Chemie (AZI)
4	I (A)	BENZOIC ACID	non-polymer		122.1	1	Chemie (BEZ)
5	K (B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
6	L, M (A, B)	water	water		18.0	369	Chemie (HOH)

Sequence modifications

A, B: 1 - 72 (UniProt: E7EX29)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag
Ala 58	Ser 58	engineered mutation

A, B: 76 - 156 (UniProt: E7EX29)

PDB	External Database	Details
Ala 73	-	linker
Ala 74	-	linker
Ala 75	-	linker
-	Val 140	deletion
-	Arg 141	deletion
-	Phe 142	deletion
-	Gly 143	deletion
-	Val 144	deletion
-	Ser 145	deletion
-	Tyr 146	deletion
-	Pro 147	deletion

A, B: 160 - 229 (UniProt: E7EX29)

PDB	External Database	Details
Ala 157	-	linker
Ala 158	-	linker
Ala 159	-	linker
Gly 230	-	linker
Gly 231	-	linker
Gly 232	-	linker
Gly 233	-	linker

A, B: 234 - 245 (UniProt: P36544)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55632.6
Non-Polymers*	Number of molecules	9
	Total formula weight	602.6
All*	Total formula weight	56235.1

*Water molecules are not included.