8P1H
Crystal structure of the chimera of human 14-3-3 zeta and phosphorylated cytoplasmic loop fragment of the alpha7 acetylcholine receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-06 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.628, 103.118, 112.804 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.060 - 1.950 |
| R-factor | 0.21099 |
| Rwork | 0.209 |
| R-free | 0.25325 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.734 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.060 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.160 | 2.857 |
| Rmeas | 0.170 | 3.023 |
| Rpim | 0.056 | 0.975 |
| Total number of observations | 525966 | 38225 |
| Number of reflections | 60333 | 4146 |
| <I/σ(I)> | 14.1 | 0.9 |
| Completeness [%] | 98.1 | |
| Redundancy | 8.7 | 9.2 |
| CC(1/2) | 0.999 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 0.2 M Magnesium acetate tetrahydrate; 0.1 M Sodium cacodylate trihydrate pH 6.5; 20% w/v Polyethylene glycol 8,000 |
