8OUW
Cryo-EM structure of CMG helicase bound to TIM-1/TIPN-1 and homodimeric DNSN-1 on fork DNA (Caenorhabditis elegans)
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (2) | DNA replication licensing factor MCM2 | polymer | 881 | 99448.6 | 1 | UniProt (Q9XXI9) Pfam (PF12619) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF23669) | Caenorhabditis elegans | |
| 2 | B (3) | DNA replication licensing factor MCM3 | polymer | 812 | 90810.7 | 1 | UniProt (Q9XVR7) Pfam (PF17855) Pfam (PF23191) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) | Caenorhabditis elegans | |
| 3 | C (4) | DNA replication licensing factor mcm-4 | polymer | 823 | 91687.6 | 1 | UniProt (Q95XQ8) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21128) | Caenorhabditis elegans | |
| 4 | D (5) | DNA replication licensing factor mcm-5 | polymer | 759 | 85057.7 | 1 | UniProt (Q21902) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF21933) | Caenorhabditis elegans | |
| 5 | E (6) | DNA replication licensing factor mcm-6 | polymer | 810 | 91248.3 | 1 | UniProt (P34647) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) Pfam (PF18263) | Caenorhabditis elegans | |
| 6 | F (7) | DNA replication licensing factor MCM7 | polymer | 730 | 81721.4 | 1 | UniProt (O16297) Pfam (PF14551) Pfam (PF17207) Pfam (PF00493) Pfam (PF17855) | Caenorhabditis elegans | |
| 7 | G (A) | Probable DNA replication complex GINS protein PSF1 | polymer | 205 | 23064.4 | 1 | UniProt (Q22019) Pfam (PF05916) Pfam (PF24997) | Caenorhabditis elegans | GINS complex subunit 1 |
| 8 | H (B) | Probable DNA replication complex GINS protein PSF2 | polymer | 180 | 20349.3 | 1 | UniProt (O62193) Pfam (PF25005) Pfam (PF05916) | Caenorhabditis elegans | GINS complex subunit 2 |
| 9 | I (C) | DNA replication complex GINS protein PSF3 | polymer | 193 | 21717.7 | 1 | UniProt (Q9BL54) Pfam (PF05916) | Caenorhabditis elegans | |
| 10 | J (D) | DNA replication complex GINS protein SLD5 | polymer | 224 | 25705.1 | 1 | UniProt (Q9U2W9) Pfam (PF05916) Pfam (PF16922) | Caenorhabditis elegans | |
| 11 | K (E) | Cell division control protein 45 homolog | polymer | 574 | 66245.8 | 1 | UniProt (Q7JMR0) Pfam (PF02724) | Caenorhabditis elegans | |
| 12 | L, M, N (F, G, H) | Downstream Neighbor of SoN homolog | polymer | 594 | 66079.4 | 3 | UniProt (H1ZUV7) | Caenorhabditis elegans | |
| 13 | O, P (I, J) | DNA Leading Strand Template | polymer | 85 | 26396.8 | 2 | Saccharomyces cerevisiae | ||
| 14 | Q (K) | Protein timeless homolog | polymer | 1353 | 157256.7 | 1 | UniProt (G5EDN3) Pfam (PF04821) Pfam (PF26019) | Caenorhabditis elegans | |
| 15 | R (L) | Protein TIPIN homolog | polymer | 237 | 27415.2 | 1 | UniProt (Q9TXI0) Pfam (PF07962) | Caenorhabditis elegans | CSM3 homolog |
| 16 | S (M) | DNA Lagging Strand Template | polymer | 61 | 18524.9 | 1 | Saccharomyces cerevisiae | ||
| 17 | AA, FA, T, Y, Z (6, 7, 2, 4, 5) | ZINC ION | non-polymer | 65.4 | 5 | Chemie (ZN) | |||
| 18 | BA, EA, U, W (6, 7, 3, 4) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) | |||
| 19 | CA, DA, V, X (6, 7, 3, 4) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 4 | Chemie (ANP) |
Sequence modifications
A: 1 - 201 (UniProt: Q22019)
L: 1 - 233 (UniProt: Q9TXI0)
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 19 |
| Total formula weight | 1151285.0 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 2449.0 | |
| All* | Total formula weight | 1153734.1 |
