8OQZ
Structure of human gamma-secretase PSEN1 APH-1B isoform reconstituted into lipid nanodisc in complex with Ab46
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Nicastrin | polymer | 717 | 79371.6 | 1 | UniProt (Q92542) Pfam (PF18266) Pfam (PF05450) | Homo sapiens (human) | |
| 2 | B (B) | Presenilin-1 CTF12 | polymer | 467 | 52669.5 | 1 | UniProt (P49768) Pfam (PF01080) | Homo sapiens (human) | PS1-CTF12 |
| 3 | C (C) | Gamma-secretase subunit APH-1B | polymer | 257 | 28480.8 | 1 | UniProt (Q8WW43) Pfam (PF06105) | Homo sapiens (human) | APH-1b,Aph-1beta,Presenilin-stabilization factor-like |
| 4 | D (D) | Gamma-secretase subunit PEN-2 | polymer | 101 | 12038.0 | 1 | UniProt (Q9NZ42) Pfam (PF10251) | Homo sapiens (human) | Presenilin enhancer protein 2 |
| 5 | E (E) | Amyloid-beta peptide 1-46 | polymer | 66 | 5634.9 | 1 | Homo sapiens (human) | ||
| 6 | F, G, H (F, G, H) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 7 | I, J, K, L, M... (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 8 | Chemie (NAG) | |||
| 8 | AA, BA, CA, DA, Q... (C, D, A, B) | 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE | non-polymer | 790.1 | 14 | Chemie (PC1) |
Sequence modifications
A: 1 - 709 (UniProt: Q92542)
B: 1 - 467 (UniProt: P49768)
| PDB | External Database | Details |
|---|---|---|
| Gly 710 | - | expression tag |
| Thr 711 | - | expression tag |
| Leu 712 | - | expression tag |
| Glu 713 | - | expression tag |
| Val 714 | - | expression tag |
| Leu 715 | - | expression tag |
| Phe 716 | - | expression tag |
| Gln 717 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 257 | Asp 257 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 178194.9 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1273.2 | |
| Non-Polymers* | Number of molecules | 22 |
| Total formula weight | 12831.7 | |
| All* | Total formula weight | 192299.8 |
