8OK9

Heterodimeric complex of Archaeoglobus fulgidus Argonaute protein Af1318 (AfAgo) with DNA and AfAgo-N protein containing N-L1-L2 domains

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Piwi protein AF_1318	polymer	441	50921.1	1	UniProt (O28951) Pfam (PF02171) In PDB	Archaeoglobus fulgidus DSM 4304	AfAgo,AfPiwi,PIWI/MID domain protein
2	C	Archaeoglobus fulgidus AfAgo-N protein	polymer	264	30575.8	1	UniProt (A0A075WKW4) In PDB	Archaeoglobus fulgidus DSM 4304
3	R, S	DNA (5'-D(P*AP*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*AP*CP*G)-3')	polymer	15	4579.0	2		Escherichia coli
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	A, C, S	ACETIC ACID	non-polymer		60.1	8	Chemie (ACY)
6	A, C, S	1,2-ETHANEDIOL	non-polymer		62.1	6	Chemie (EDO)
7	C	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
8		water	water		18.0	200	Chemie (HOH)

Sequence modifications

A: 1 - 427 (UniProt: O28951)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

C: 1 - 250 (UniProt: A0A075WKW4)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	90654.9
Non-Polymers*	Number of molecules	16
	Total formula weight	983.2
All*	Total formula weight	91638.1

*Water molecules are not included.