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8OK9

Heterodimeric complex of Archaeoglobus fulgidus Argonaute protein Af1318 (AfAgo) with DNA and AfAgo-N protein containing N-L1-L2 domains

Entity
Entity IDChain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
1A
(A)
Piwi protein AF_1318polymer44150921.11UniProt (O28951)
Pfam (PF02171)
Archaeoglobus fulgidus DSM 4304AfAgo,AfPiwi,PIWI/MID domain protein
2B
(C)
Archaeoglobus fulgidus AfAgo-N proteinpolymer26430575.81UniProt (A0A075WKW4)Archaeoglobus fulgidus DSM 4304
3C, D
(R, S)
DNA (5'-D(P*AP*TP*CP*GP*AP*CP*CP*AP*GP*GP*CP*TP*AP*CP*G)-3')polymer154579.02Escherichia coli
4E
(A)
MAGNESIUM IONnon-polymer24.31Chemie (MG)
5F, G, H, I, J...
(A, C, S)
ACETIC ACIDnon-polymer60.18Chemie (ACY)
6K, L, M, P, S...
(A, C, S)
1,2-ETHANEDIOLnon-polymer62.16Chemie (EDO)
7Q
(C)
DI(HYDROXYETHYL)ETHERnon-polymer106.11Chemie (PEG)
8U, V, W, X
(A, C, R, S)
waterwater18.0200Chemie (HOH)
Sequence modifications
A: 1 - 427 (UniProt: O28951)
PDBExternal DatabaseDetails
Met -13-initiating methionine
Gly -12-expression tag
Ser -11-expression tag
Ser -10-expression tag
His -9-expression tag
His -8-expression tag
His -7-expression tag
His -6-expression tag
His -5-expression tag
His -4-expression tag
Ser -3-expression tag
Gln -2-expression tag
Asp -1-expression tag
Pro 0-expression tag
C: 1 - 250 (UniProt: A0A075WKW4)
PDBExternal DatabaseDetails
Met -13-initiating methionine
Gly -12-expression tag
Ser -11-expression tag
Ser -10-expression tag
His -9-expression tag
His -8-expression tag
His -7-expression tag
His -6-expression tag
His -5-expression tag
His -4-expression tag
Ser -3-expression tag
Gln -2-expression tag
Asp -1-expression tag
Pro 0-expression tag
Sequence viewer
Contents of the asymmetric unit
PolymersNumber of chains4
Total formula weight90654.9
Non-Polymers*Number of molecules16
Total formula weight983.2
All*Total formula weight91638.1
*Water molecules are not included.

246031

PDB entries from 2025-12-10

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