8OK9
Heterodimeric complex of Archaeoglobus fulgidus Argonaute protein Af1318 (AfAgo) with DNA and AfAgo-N protein containing N-L1-L2 domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-25 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 1.01000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.714, 105.893, 144.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 64.690 - 2.500 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.22690 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.876 |
Data reduction software | XDS (VERSION Jun 17, 2015) |
Data scaling software | Aimless (version 0.7.2 : 27/05/18) |
Phasing software | MOLREP (version 6.1 : 23/01/06) |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 144.350 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.054 | 0.413 |
Rmeas | 0.058 | 0.446 |
Rpim | 0.022 | 0.167 |
Number of reflections | 44100 | 4576 |
<I/σ(I)> | 30.2 | 6.6 |
Completeness [%] | 98.1 | 100 |
Redundancy | 13.3 | 13.7 |
CC(1/2) | 0.999 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 280 | BisTris pH 5.5 0.1 M;Ammonium acetate 0.1 M; PEG10k 8%; cryo-cooling with addition of ethylene glycol to 30 % |