8OK2

Bipartite interaction of TOPBP1 with the GINS complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA replication complex GINS protein PSF1	polymer	151	17772.4	1	UniProt (Q14691) Pfam (PF05916)	Homo sapiens (human)	GINS complex subunit 1
2	B (B)	DNA replication complex GINS protein PSF2	polymer	185	21453.7	1	UniProt (Q9Y248) Pfam (PF25005) Pfam (PF05916)	Homo sapiens (human)	GINS complex subunit 2
3	C (C)	DNA replication complex GINS protein PSF3	polymer	216	24562.6	1	UniProt (Q9BRX5) Pfam (PF22466) Pfam (PF05916)	Homo sapiens (human)	GINS complex subunit 3
4	D (D)	DNA replication complex GINS protein SLD5	polymer	262	30831.8	1	UniProt (Q9BRT9) Pfam (PF05916) Pfam (PF16922)	Homo sapiens (human)	GINS complex subunit 4
5	E (E)	Topoisomerase (DNA) II binding protein 1	polymer	470	52397.5	1	UniProt (A7E2X7) Pfam (PF00533) UniProt (by SIFTS) (Q92547)	Homo sapiens (human)

Sequence modifications

A: 1 - 151 (UniProt: Q14691)

PDB	External Database	Details
Ile 97	Val 97	engineered mutation

D: 1 - 223 (UniProt: Q9BRT9)

PDB	External Database	Details
Met -38	-	initiating methionine
His -37	-	expression tag
His -36	-	expression tag
His -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
Gly -31	-	expression tag
Arg -30	-	expression tag
Met -29	-	expression tag
Asp -28	-	expression tag
Tyr -27	-	expression tag
Lys -26	-	expression tag
Asp -25	-	expression tag
Asp -24	-	expression tag
Asp -23	-	expression tag
Asp -22	-	expression tag
Lys -21	-	expression tag
Ala -20	-	expression tag
Asp -19	-	expression tag
Tyr -18	-	expression tag
Lys -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Lys -12	-	expression tag
Ala -11	-	expression tag
Asp -10	-	expression tag
Tyr -9	-	expression tag
Lys -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
Pro 0	-	expression tag

E: 331 - 766 (UniProt: A7E2X7)

PDB	External Database	Details
Met 308	-	initiating methionine
Gly 309	-	expression tag
Ser 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
Gly 317	-	expression tag
Ser 318	-	expression tag
Leu 319	-	expression tag
Glu 320	-	expression tag
Val 321	-	expression tag
Leu 322	-	expression tag
Phe 323	-	expression tag
Gln 324	-	expression tag
Gly 325	-	expression tag
Pro 326	-	expression tag
His 327	-	expression tag
Met 328	-	expression tag
Ala 329	-	expression tag
Ser 330	-	expression tag
Arg 767	-	expression tag
Arg 768	-	expression tag
Ala 769	-	expression tag
Trp 770	-	expression tag
Ser 771	-	expression tag
His 772	-	expression tag
Pro 773	-	expression tag
Gln 774	-	expression tag
Phe 775	-	expression tag
Glu 776	-	expression tag
Lys 777	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	147018.1
All*	Total formula weight	147018.1

*Water molecules are not included.