8KAQ

Glycoside hydrolase family 1 beta-glucosidase (E318G mutant) from Streptomyces griseus (sophorose complex)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Putative beta-glucosidase	polymer	414	45690.6	4	UniProt (B1W1N0) Pfam (PF00232) In PDB	Streptomyces griseus subsp. griseus NBRC 13350
2	A, B, C, D	beta-D-glucopyranose	non-polymer		180.2	8	Chemie (BGC)
3	A, B, C	TRIETHYLENE GLYCOL	non-polymer		150.2	3	Chemie (PGE)
4	A, B, C	alpha-D-glucopyranose	non-polymer		180.2	3	Chemie (GLC)
5	A, B, C, D	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
6		water	water		18.0	529	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 406 (UniProt: B1W1N0)

PDB	External Database	Details
Gly 318	Glu 318	engineered mutation
Leu 407	-	expression tag
Glu 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag
His 414	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	182762.4
Non-Polymers*	Number of molecules	20
	Total formula weight	2645.0
All*	Total formula weight	185407.4

*Water molecules are not included.