8KAQ
Glycoside hydrolase family 1 beta-glucosidase (E318G mutant) from Streptomyces griseus (sophorose complex)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.793, 100.179, 96.048 |
Unit cell angles | 90.00, 102.87, 90.00 |
Refinement procedure
Resolution | 46.824 - 2.130 |
Rwork | 0.183 |
R-free | 0.22330 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.372 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.824 | 2.170 |
High resolution limit [Å] | 2.130 | 2.130 |
Number of reflections | 93387 | 4558 |
<I/σ(I)> | 10.4 | |
Completeness [%] | 98.7 | |
Redundancy | 3.4 | |
CC(1/2) | 0.995 | 0.637 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris propane (pH 6.5), 0.3 M sodium formate, 18% PEG3350 |