8K3Y

The "5+1" heteromeric structure of Lon protease consisting of a spiral pentamer with Y224S mutation and an N-terminal-truncated monomeric E613K mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Lon protease	polymer	799	89307.4	5	UniProt (A0A059VAZ3)	Meiothermus taiwanensis	ATP-dependent protease La
2	F (F)	Lon protease	polymer	570	63185.3	1	UniProt (A0A059VAZ3)	Meiothermus taiwanensis	ATP-dependent protease La
3	G, H, I, J (B, C, D, E)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)

Sequence modifications

A, B, C, D, E: 1 - 793 (UniProt: A0A059VAZ3)

PDB	External Database	Details
Ser 224	Tyr 224	engineered mutation
His 794	-	expression tag
His 795	-	expression tag
His 796	-	expression tag
His 797	-	expression tag
His 798	-	expression tag
His 799	-	expression tag

F: 242 - 793 (UniProt: A0A059VAZ3)

PDB	External Database	Details
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
Ser 230	-	expression tag
Ser 231	-	expression tag
Gly 232	-	expression tag
Glu 233	-	expression tag
Asn 234	-	expression tag
Leu 235	-	expression tag
Tyr 236	-	expression tag
Phe 237	-	expression tag
Gln 238	-	expression tag
Gly 239	-	expression tag
His 240	-	expression tag
Met 241	-	expression tag
Lys 613	Glu 613	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	509722.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1708.8
All*	Total formula weight	511431.0

*Water molecules are not included.