8JRV

Cryo-EM structure of the glucagon receptor bound to glucagon and beta-arrestin 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	HA signal peptide,HPC4 purification tag,Glucagon receptor,C-terminal tail of Vasopressin V2 receptor	polymer	476	54163.0	1	UniProt (P03435) UniProt (P04070) UniProt (P47871) UniProt (P30518) Pfam (PF02793) Pfam (PF00002)	Influenza A virus (strain A/Victoria/3/1975 H3N2)	GL-R,V2R,AVPR V2,Antidiuretic hormone receptor,Renal-type arginine vasopressin receptor
2	B (G)	Glucagon	polymer	29	3486.8	1	UniProt (P01275) Pfam (PF00123)	Homo sapiens
3	C, D, E (A, H, L)	Beta-arrestin 1 and single-chain fragment variable 30 (scFv30)	polymer	627	69173.9	3	Pfam (PF00339) Pfam (PF02752) UniProt (by SIFTS) (P49407)	Homo sapiens
4	F (B)	Nanobody 32	polymer	126	13867.4	1		Escherichia phage EcSzw-2
5	G (A)	[(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate	non-polymer		746.6	1	Chemie (PIO)

Sequence modifications

R: -14 - 1 (UniProt: P03435)

PDB	External Database	Details
Gly 2	-	linker
Ala 3	-	linker
Pro 4	-	linker

R: 5 - 16 (UniProt: P04070)

PDB	External Database	Details
Gly 17	-	linker
Ser 18	-	linker
Gly 19	-	linker
Ser 20	-	linker
Ala 21	-	linker
Gly 22	-	linker
Ser 23	-	linker
Ala 24	-	linker
Gly 25	-	linker
Ser 26	-	linker

R: 27 - 1342 (UniProt: P47871)
R: 1343 - 1371 (UniProt: P30518)

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	279038.9
Non-Polymers*	Number of molecules	1
	Total formula weight	746.6
All*	Total formula weight	279785.4

*Water molecules are not included.