8JPX
Cryo-EM structure of PfAgo-guide DNA-target DNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (B, A) | Protein argonaute | polymer | 770 | 90510.1 | 2 | UniProt (Q8U3D2) Pfam (PF22476) Pfam (PF12212) Pfam (PF02171) | Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) | PfAgo |
| 2 | B, E (H, G) | Guide DNA | polymer | 17 | 5337.5 | 2 | Pyrococcus furiosus DSM 3638 | ||
| 3 | C, F (U, T) | Target DNA | polymer | 17 | 5075.3 | 2 | Pyrococcus furiosus DSM 3638 | ||
| 4 | G, H (E, F) | Excess DNA | polymer | 6 | 1780.2 | 2 | Pyrococcus furiosus DSM 3638 | ||
| 5 | I, J, K, L, M... (B, H, A, G) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) |
Sequence modifications
B, A: 1 - 770 (UniProt: Q8U3D2)
| PDB | External Database | Details |
|---|---|---|
| Ile 4 | Lys 4 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 205406.2 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 145.8 | |
| All* | Total formula weight | 205552.0 |
