8JO4
Cryo-EM structure of a Legionella effector complexed with actin and ATP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Substrate of the Dot/Icm secretion system | polymer | 433 | 50352.2 | 1 | UniProt (A0A2H5FI52) | Legionella sainthelensi | |
| 2 | B (B) | Actin, alpha skeletal muscle | polymer | 377 | 42097.0 | 1 | UniProt (P68135) Pfam (PF00022) | Oryctolagus cuniculus (rabbit) | Alpha-actin-1 |
| 3 | C, F (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 4 | D (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | E (B) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) |
Sequence modifications
A: 1 - 426 (UniProt: A0A2H5FI52)
| PDB | External Database | Details |
|---|---|---|
| Ala 287 | His 287 | engineered mutation |
| Glu 427 | - | expression tag |
| His 428 | - | expression tag |
| His 429 | - | expression tag |
| His 430 | - | expression tag |
| His 431 | - | expression tag |
| His 432 | - | expression tag |
| His 433 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 92449.2 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1078.7 | |
| All* | Total formula weight | 93527.9 |
