8JO4

Cryo-EM structure of a Legionella effector complexed with actin and ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Substrate of the Dot/Icm secretion system	polymer	433	50352.2	1	UniProt (A0A2H5FI52) In PDB	Legionella sainthelensi
2	B	Actin, alpha skeletal muscle	polymer	377	42097.0	1	UniProt (P68135) Pfam (PF00022) In PDB	Oryctolagus cuniculus (rabbit)	Alpha-actin-1
3	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
4	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	B	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)

Sequence modifications

A: 1 - 426 (UniProt: A0A2H5FI52)

PDB	External Database	Details
Ala 287	His 287	engineered mutation
Glu 427	-	expression tag
His 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	92449.2
Non-Polymers*	Number of molecules	4
	Total formula weight	1078.7
All*	Total formula weight	93527.9

*Water molecules are not included.