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- EMDB-36455: Cryo-EM structure of a Legionella effector complexed with actin a... -
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Open data
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Basic information
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Title | Cryo-EM structure of a Legionella effector complexed with actin and ATP | |||||||||||||||
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Function / homology | ![]() cytoskeletal motor activator activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() ![]() ![]() | |||||||||||||||
Method | ![]() ![]() | |||||||||||||||
![]() | Zhou XT / Wang XF / Tan JX / Zhu YQ | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Cryo-EM structure of a Legionella effector complexed with actin and ATP Authors: Zhou XT / Wang XF / Tan JX / Zhu YQ | |||||||||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 59.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 16.6 KB 16.6 KB | Display Display | ![]() |
Images | ![]() | 48 KB | ||
Filedesc metadata | ![]() | 6.4 KB | ||
Others | ![]() ![]() | 59.4 MB 59.4 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8jo4MC M: atomic model generated by this map C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
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Voxel size | X=Y=Z: 0.93 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #2
File | emd_36455_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_36455_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Cryo-EM structure of a Legionella effector complexed with actin a...
Entire | Name: Cryo-EM structure of a Legionella effector complexed with actin and ATP |
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Components |
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-Supramolecule #1: Cryo-EM structure of a Legionella effector complexed with actin a...
Supramolecule | Name: Cryo-EM structure of a Legionella effector complexed with actin and ATP type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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Source (natural) | Organism: ![]() |
-Macromolecule #1: Substrate of the Dot/Icm secretion system
Macromolecule | Name: Substrate of the Dot/Icm secretion system / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO |
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Source (natural) | Organism: ![]() |
Molecular weight | Theoretical: 50.352203 KDa |
Recombinant expression | Organism: ![]() ![]() ![]() |
Sequence | String: MSYTKIESSL LLALDYPKLD ESDFILLLTK FLEKKLGNND NYPTFSKQIQ KYYLEQEYKK AIENILRLCQ ENETLLGTNL VQRLITKSS QVTSNPKDNE SRRFYEVLYA EHLESILRKD FDCSIFDELN EAYNEVRPEY TVNDLTKINT FEEARKLILA F VMLNDNVE ...String: MSYTKIESSL LLALDYPKLD ESDFILLLTK FLEKKLGNND NYPTFSKQIQ KYYLEQEYKK AIENILRLCQ ENETLLGTNL VQRLITKSS QVTSNPKDNE SRRFYEVLYA EHLESILRKD FDCSIFDELN EAYNEVRPEY TVNDLTKINT FEEARKLILA F VMLNDNVE LGLKAQSAIY QKKDRSREEL GQVLTANPGI MKPNSPNFAD NTVPIKKIDK IAIDEKKAGG YSKTNPQVPF VA SLSGTTY SLVVVLQKYM DKHKTDPNLE KKINNIVMLW TSAYIKDGYA SYKEVIDIFK DAHIQSIFAR ANIKLDYAII DDT DHEFHR AQEYTQGIAT KAMMHQELVQ KVQEKSMREE KQKQMLLFCG VLVNQLNQDP KSKEKYPEQL ESLNALYKNW NAGT IKNQE FKKECTQVCT EIQIKEQNTP LEHHHHHH UniProtKB: Substrate of the Dot/Icm secretion system |
-Macromolecule #2: Actin, alpha skeletal muscle
Macromolecule | Name: Actin, alpha skeletal muscle / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO EC number: ![]() |
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Source (natural) | Organism: ![]() ![]() ![]() |
Molecular weight | Theoretical: 42.096953 KDa |
Sequence | String: MCDEDETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWH HTFYNELRVA PEEHPTLLTE APLNPKANRE KMTQIMFETF NVPAMYVAIQ AVLSLYASGR TTGIVLDSGD G VTHNVPIY ...String: MCDEDETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWH HTFYNELRVA PEEHPTLLTE APLNPKANRE KMTQIMFETF NVPAMYVAIQ AVLSLYASGR TTGIVLDSGD G VTHNVPIY EGYALPHAIM RLDLAGRDLT DYLMKILTER GYSFVTTAER EIVRDIKEKL CYVALDFENE MATAASSSSL EK SYELPDG QVITIGNERF RCPETLFQPS FIGMESAGIH ETTYNSIMKC DIDIRKDLYA NNVMSGGTTM YPGIADRMQK EIT ALAPST MKIKIIAPPE RKYSVWIGGS ILASLSTFQQ MWITKQEYDE AGPSIVHRKC F UniProtKB: ![]() |
-Macromolecule #3: ADENOSINE-5'-TRIPHOSPHATE
Macromolecule | Name: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 2 / Formula: ATP |
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Molecular weight | Theoretical: 507.181 Da |
Chemical component information | ![]() ChemComp-ATP: |
-Macromolecule #4: MAGNESIUM ION
Macromolecule | Name: MAGNESIUM ION / type: ligand / ID: 4 / Number of copies: 1 / Formula: MG |
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Molecular weight | Theoretical: 24.305 Da |
-Macromolecule #5: CALCIUM ION
Macromolecule | Name: CALCIUM ION / type: ligand / ID: 5 / Number of copies: 1 / Formula: CA |
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Molecular weight | Theoretical: 40.078 Da |
-Experimental details
-Structure determination
Method | ![]() |
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Aggregation state | particle |
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Sample preparation
Buffer | pH: 8 |
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Grid | Model: Quantifoil R0.6/1 / Material: COPPER / Mesh: 300 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 180 sec. |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD![]() |
Image recording | Film or detector model: FEI FALCON IV (4k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
Startup model | Type of model: NONE |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.04 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 171800 |
-Atomic model buiding 1
Initial model | Chain - Source name: Other / Chain - Initial model type: in silico model / Details: ModelAngelo built model |
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Output model | ![]() PDB-8jo4: |