8JLA

Cryo-EM structure of the human nucleosome lacking N-terminal region of H2A, H2B, H3, and H4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.1	polymer	112	12817.0	2	UniProt (P68431) Pfam (PF00125)	Homo sapiens (human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, F (B, F)	Histone H4	polymer	91	10404.2	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (human)
3	C, G (C, G)	Histone H2A type 1-B/E	polymer	124	13588.9	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (human)	Histone H2A.2,Histone H2A/a,Histone H2A/m
4	D, H (D, H)	Histone H2B type 1-J	polymer	105	11751.5	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (human)	Histone H2B.1,Histone H2B.r,H2B/r
5	I (I)	DNA (193-MER)	polymer	193	59590.0	1		synthetic construct
6	J (J)	DNA (193-MER)	polymer	193	59581.0	1		synthetic construct

Sequence modifications

A, E: 28 - 135 (UniProt: P68431)

PDB	External Database	Details
Gly 24	-	expression tag
Ser 25	-	expression tag
His 26	-	expression tag
Met 27	-	expression tag

B, F: 16 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly 12	-	expression tag
Ser 13	-	expression tag
His 14	-	expression tag
Met 15	-	expression tag

C, G: 10 - 129 (UniProt: P04908)

PDB	External Database	Details
Gly 6	-	expression tag
Ser 7	-	expression tag
His 8	-	expression tag
Met 9	-	expression tag

D, H: 25 - 125 (UniProt: P06899)

PDB	External Database	Details
Gly 21	-	expression tag
Ser 22	-	expression tag
His 23	-	expression tag
Met 24	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	216294.2
All*	Total formula weight	216294.2

*Water molecules are not included.