8JLA
Cryo-EM structure of the human nucleosome lacking N-terminal region of H2A, H2B, H3, and H4
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.44 |
| Software | RELION |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
