8JKB

Cryo-EM structure of KCTD5 in complex with Gbeta gamma subunits

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, J, L, N (B, F, I, J, K)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	372	41088.8	5	UniProt (P62873) Pfam (PF25391)	Homo sapiens (human)
2	B, D, K, M, O (G, L, M, N, O)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	96	10228.7	5	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	E, F, G, H, I (A, H, C, D, E)	BTB/POZ domain-containing protein KCTD5	polymer	274	30780.6	5	UniProt (Q8VC57) Pfam (PF02214)	Mus musculus (house mouse)

Sequence modifications

B, F, I, J, K: 1 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -31	-	initiating methionine
Tyr -30	-	expression tag
Pro -29	-	expression tag
Tyr -28	-	expression tag
Asp -27	-	expression tag
Val -26	-	expression tag
Pro -25	-	expression tag
Asp -24	-	expression tag
Tyr -23	-	expression tag
Ala -22	-	expression tag
Tyr -21	-	expression tag
Pro -20	-	expression tag
Tyr -19	-	expression tag
Asp -18	-	expression tag
Val -17	-	expression tag
Pro -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Ala -13	-	expression tag
Tyr -12	-	expression tag
Pro -11	-	expression tag
Tyr -10	-	expression tag
Asp -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Ala -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

G, L, M, N, O: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Gly -24	-	expression tag
Ser -23	-	expression tag
Ala -22	-	expression tag
Leu -21	-	expression tag
Glu -20	-	expression tag
Val -19	-	expression tag
Leu -18	-	expression tag
Phe -17	-	expression tag
Gln -16	-	expression tag
Gly -15	-	expression tag
Pro -14	-	expression tag
Gly -13	-	expression tag
Ala -12	-	expression tag
Gly -11	-	expression tag
Ala -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Val -7	-	expression tag
Leu -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ser 68	Cys 68	engineered mutation

A, H, C, D, E: 1 - 234 (UniProt: Q8VC57)

PDB	External Database	Details
Met -39	-	initiating methionine
Gly -38	-	expression tag
Ser -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
His -34	-	expression tag
His -33	-	expression tag
His -32	-	expression tag
His -31	-	expression tag
His -30	-	expression tag
Gly -29	-	expression tag
Ser -28	-	expression tag
Glu -27	-	expression tag
Asp -26	-	expression tag
Gln -25	-	expression tag
Val -24	-	expression tag
Asp -23	-	expression tag
Pro -22	-	expression tag
Arg -21	-	expression tag
Leu -20	-	expression tag
Ile -19	-	expression tag
Asp -18	-	expression tag
Gly -17	-	expression tag
Lys -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Lys -13	-	expression tag
Asp -12	-	expression tag
Asp -11	-	expression tag
Asp -10	-	expression tag
Asp -9	-	expression tag
Lys -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	410490.6
All*	Total formula weight	410490.6

*Water molecules are not included.