8J22

Cryo-EM structure of FFAR2 complex bound with TUG-1375

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
2	B	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096) Pfam (PF00503) In PDB	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
3	C	Free fatty acid receptor 2	polymer	311	35184.6	1	UniProt (O15552) Pfam (PF00001) In PDB	Homo sapiens (human)	G-protein coupled receptor 43
4	D	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I
5	F	scFV16	polymer	297	31870.6	1		Homo sapiens
6	C	(2R,4R)-2-(2-chlorophenyl)-3-[4-(3,5-dimethyl-1,2-oxazol-4-yl)phenyl]carbonyl-1,3-thiazolidine-4-carboxylic acid	non-polymer		442.9	1	Chemie (9UJ)

Sequence modifications

A: 0 - 338 (UniProt: P62873)

PDB	External Database	Details
Met -12	-	initiating methionine
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Leu -3	-	expression tag
Leu -2	-	expression tag
Gln -1	-	expression tag
Gly 339	-	expression tag
Ser 340	-	expression tag
Ser 341	-	expression tag
Gly 342	-	expression tag
Gly 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Ser 346	-	expression tag
Gly 347	-	expression tag
Gly 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Ser 352	-	expression tag
Gly 353	-	expression tag
Val 354	-	expression tag
Ser 355	-	expression tag
Gly 356	-	expression tag
Trp 357	-	expression tag
Arg 358	-	expression tag
Leu 359	-	expression tag
Phe 360	-	expression tag
Lys 361	-	expression tag
Lys 362	-	expression tag
Ile 363	-	expression tag
Ser 364	-	expression tag

C: 1 - 311 (UniProt: O15552)

PDB	External Database	Details
Thr 2	Leu 2	conflict
Val 35	Arg 35	conflict
Val 36	Ile 36	conflict
Ile 80	Val 80	variant
Gly 86	Ser 86	conflict
Asn 158	Ser 158	variant
Glu 171	Asn 171	conflict
Thr 208	Ser 208	conflict
His 211	Leu 211	variant
Phe 246	Tyr 246	variant
Tyr 247	His 247	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	156387.2
Non-Polymers*	Number of molecules	1
	Total formula weight	442.9
All*	Total formula weight	156830.1

*Water molecules are not included.