8ITF
Cryo-EM structure of the DMCHA-bound mTAAR9-Gs complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A (A) | Guanine nucleotide-binding protein G(s) subunit alpha isoforms short | polymer | 362 | 42010.7 | 1 | Homo sapiens (human) | ||
2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 377 | 41055.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | |
3 | C (C) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 58 | 6375.3 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
4 | D (R) | Trace amine-associated receptor 9 | polymer | 348 | 38928.2 | 1 | UniProt (Q5QD04) Pfam (PF00001) | Mus musculus (house mouse) | TaR-9,Trace amine receptor 9,mTaar9 |
5 | E (S) | scFv16 | polymer | 285 | 30363.0 | 1 | synthetic construct | ||
6 | F (N) | Nanobody-35 | polymer | 128 | 13885.4 | 1 | synthetic construct | ||
7 | G (R) | ~{N},~{N}-dimethylcyclohexanamine | non-polymer | 127.2 | 1 | Chemie (8IA) |
Sequence modifications
B: 2 - 340 (UniProt: P62873)
PDB | External Database | Details |
---|---|---|
Met -10 | - | initiating methionine |
His -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
Gly -3 | - | expression tag |
Ser -2 | - | expression tag |
Leu -1 | - | expression tag |
Leu 0 | - | expression tag |
Gln 1 | - | expression tag |
Gly 341 | - | expression tag |
Ser 342 | - | expression tag |
Ser 343 | - | expression tag |
Gly 344 | - | expression tag |
Gly 345 | - | expression tag |
Gly 346 | - | expression tag |
Gly 347 | - | expression tag |
Ser 348 | - | expression tag |
Gly 349 | - | expression tag |
Gly 350 | - | expression tag |
Gly 351 | - | expression tag |
Gly 352 | - | expression tag |
Ser 353 | - | expression tag |
Ser 354 | - | expression tag |
Gly 355 | - | expression tag |
Val 356 | - | expression tag |
Ser 357 | - | expression tag |
Gly 358 | - | expression tag |
Trp 359 | - | expression tag |
Arg 360 | - | expression tag |
Leu 361 | - | expression tag |
Phe 362 | - | expression tag |
Lys 363 | - | expression tag |
Lys 364 | - | expression tag |
Ile 365 | - | expression tag |
Ser 366 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 172618.6 | |
Non-Polymers* | Number of molecules | 1 |
Total formula weight | 127.2 | |
All* | Total formula weight | 172745.8 |