8IMU
Dihydroxyacid dehydratase (DHAD) mutant-V497F
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B | Dihydroxy-acid dehydratase, chloroplastic | polymer | 574 | 61186.9 | 2 | UniProt (Q9LIR4) Pfam (PF00920) In PDB | Arabidopsis thaliana (thale cress) | AthDHAD,DAD |
| 2 | A, B | FE2/S2 (INORGANIC) CLUSTER | non-polymer | 175.8 | 2 | Chemie (FES) | |||
| 3 | A, B | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 4 | A, B | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | water | water | 18.0 | 594 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 574 (UniProt: Q9LIR4)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Phe 497 | Val 531 | engineered mutation |
| Ala 525 | Lys 559 | engineered mutation |
| Ala 526 | Lys 560 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 122373.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 653.9 | |
| All* | Total formula weight | 123027.6 |
