8IMU
Dihydroxyacid dehydratase (DHAD) mutant-V497F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-12 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.97892 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 135.120, 135.120, 136.440 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.000 - 1.930 |
R-factor | 0.1829 |
Rwork | 0.182 |
R-free | 0.20780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ze4 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.038 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 1.960 |
High resolution limit [Å] | 1.930 | 1.930 |
Rmerge | 0.108 | 1.473 |
Number of reflections | 95022 | 4650 |
<I/σ(I)> | 13.6 | 2 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 13.2 | 13.4 |
CC(1/2) | 0.999 | 0.870 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 0.1 M sodium acetate pH 5.0 1.5 M ammonium sulfate |