8IMU

Dihydroxyacid dehydratase (DHAD) mutant-V497F

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0004160	molecular_function	dihydroxy-acid dehydratase activity
A	0005507	molecular_function	copper ion binding
A	0005829	cellular_component	cytosol
A	0008652	biological_process	amino acid biosynthetic process
A	0009082	biological_process	branched-chain amino acid biosynthetic process
A	0009097	biological_process	isoleucine biosynthetic process
A	0009099	biological_process	valine biosynthetic process
A	0009507	cellular_component	chloroplast
A	0009536	cellular_component	plastid
A	0009553	biological_process	embryo sac development
A	0009555	biological_process	pollen development
A	0009570	cellular_component	chloroplast stroma
A	0009651	biological_process	response to salt stress
A	0016829	molecular_function	lyase activity
A	0016836	molecular_function	hydro-lyase activity
A	0046872	molecular_function	metal ion binding
A	0048364	biological_process	root development
A	0051537	molecular_function	2 iron, 2 sulfur cluster binding
B	0003824	molecular_function	catalytic activity
B	0004160	molecular_function	dihydroxy-acid dehydratase activity
B	0005507	molecular_function	copper ion binding
B	0005829	cellular_component	cytosol
B	0008652	biological_process	amino acid biosynthetic process
B	0009082	biological_process	branched-chain amino acid biosynthetic process
B	0009097	biological_process	isoleucine biosynthetic process
B	0009099	biological_process	valine biosynthetic process
B	0009507	cellular_component	chloroplast
B	0009536	cellular_component	plastid
B	0009553	biological_process	embryo sac development
B	0009555	biological_process	pollen development
B	0009570	cellular_component	chloroplast stroma
B	0009651	biological_process	response to salt stress
B	0016829	molecular_function	lyase activity
B	0016836	molecular_function	hydro-lyase activity
B	0046872	molecular_function	metal ion binding
B	0048364	biological_process	root development
B	0051537	molecular_function	2 iron, 2 sulfur cluster binding

Functional Information from PROSITE/UniProt

site_id	PS00886
Number of Residues	11
Details	ILVD_EDD_1 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 1. CDKnmPGtimA

Chain	Residue	Details
A	CYS139-ALA149

---

site_id	PS00887
Number of Residues	12
Details	ILVD_EDD_2 Dihydroxy-acid and 6-phosphogluconate dehydratases signature 2. ALLTDGRFSGGS

Chain	Residue	Details
A	ALA481-SER492

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Proton acceptor => ECO:0000250|UniProtKB:P9WKJ5

Chain	Residue	Details
A	SER489
B	SER489

---

site_id	SWS_FT_FI2
Number of Residues	10
Details	BINDING: BINDING => ECO:0000269|PubMed:29995859, ECO:0007744|PDB:5ZE4

Chain	Residue	Details
A	CYS66
B	GLU463
A	CYS139
A	ASP140
A	CYS211
A	GLU463
B	CYS66
B	CYS139
B	ASP140
B	CYS211

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250|UniProtKB:P9WKJ5

Chain	Residue	Details
A	ASP98
B	ASP98

---