8HVR

Cryo-EM structure of AfsR-dependent transcription activation complex with afsS promoter

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, K (A, B, K)	DNA-directed RNA polymerase subunit alpha	polymer	340	36734.6	3	UniProt (A0A6G2M9E1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P60312)	Streptomyces coelicolor A3(2)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C (C)	DNA-directed RNA polymerase subunit beta	polymer	1161	128644.9	1	UniProt (Q9L0L0) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560)	Streptomyces coelicolor A3(2)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D (D)	DNA-directed RNA polymerase subunit beta'	polymer	1307	145912.2	1	UniProt (Q8CJT1) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998)	Streptomyces coelicolor A3(2)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E (E)	DNA-directed RNA polymerase subunit omega	polymer	90	9716.9	1	UniProt (Q9KXS1) Pfam (PF01192)	Streptomyces coelicolor A3(2)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F (F)	RNA polymerase principal sigma factor HrdB	polymer	531	58189.0	1	UniProt (P18183) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545)	Streptomyces coelicolor A3(2)	RNA polymerase sigma factor SigA
6	G (G)	RNA polymerase-binding protein RbpA	polymer	124	14146.1	1	UniProt (A0A6M9XI42) Pfam (PF13397) UniProt (by SIFTS) (Q9RKY0)	Streptomyces coelicolor A3(2)
7	H (H)	Putative transcriptional factor regulator	polymer	160	17855.3	1	UniProt (Q9L0Q9) Pfam (PF02559) Pfam (PF21095)	Streptomyces coelicolor A3(2)
8	I, J (I, J)	Regulatory protein AfsR	polymer	290	31479.6	2	UniProt (P25941) Pfam (PF00486) Pfam (PF03704)	Streptomyces coelicolor A3(2)
9	L (O)	DNA (65-MER)	polymer	65	19863.7	1		Streptomyces coelicolor A3(2)
10	M (P)	DNA (65-MER)	polymer	65	20086.8	1		Streptomyces coelicolor A3(2)
11	N (D)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
12	O, P (D)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

D: 1 - 1299 (UniProt: Q8CJT1)

PDB	External Database	Details
His 1300	-	expression tag
His 1301	-	expression tag
His 1302	-	expression tag
His 1303	-	expression tag
His 1304	-	expression tag
His 1305	-	expression tag
His 1306	-	expression tag
His 1307	-	expression tag

F: 1 - 511 (UniProt: P18183)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

I, J: 1 - 270 (UniProt: P25941)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	13
	Total formula weight	587578.1
Non-Polymers*	Number of molecules	3
	Total formula weight	155.1
All*	Total formula weight	587733.2

*Water molecules are not included.