8HVR
Cryo-EM structure of AfsR-dependent transcription activation complex with afsS promoter
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, K | DNA-directed RNA polymerase subunit alpha | polymer | 340 | 36734.6 | 3 | UniProt (A0A6G2M9E1) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P60312) In PDB | Streptomyces coelicolor A3(2) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
2 | C | DNA-directed RNA polymerase subunit beta | polymer | 1161 | 128644.9 | 1 | UniProt (Q9L0L0) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB | Streptomyces coelicolor A3(2) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
3 | D | DNA-directed RNA polymerase subunit beta' | polymer | 1307 | 145912.2 | 1 | UniProt (Q8CJT1) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Streptomyces coelicolor A3(2) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
4 | E | DNA-directed RNA polymerase subunit omega | polymer | 90 | 9716.9 | 1 | UniProt (Q9KXS1) Pfam (PF01192) In PDB | Streptomyces coelicolor A3(2) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
5 | F | RNA polymerase principal sigma factor HrdB | polymer | 531 | 58189.0 | 1 | UniProt (P18183) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Streptomyces coelicolor A3(2) | RNA polymerase sigma factor SigA |
6 | G | RNA polymerase-binding protein RbpA | polymer | 124 | 14146.1 | 1 | UniProt (A0A6M9XI42) Pfam (PF13397) UniProt (by SIFTS) (Q9RKY0) In PDB | Streptomyces coelicolor A3(2) | |
7 | H | Putative transcriptional factor regulator | polymer | 160 | 17855.3 | 1 | UniProt (Q9L0Q9) Pfam (PF02559) Pfam (PF21095) In PDB | Streptomyces coelicolor A3(2) | |
8 | I, J | Regulatory protein AfsR | polymer | 290 | 31479.6 | 2 | UniProt (P25941) Pfam (PF00486) Pfam (PF03704) In PDB | Streptomyces coelicolor A3(2) | |
9 | O | DNA (65-MER) | polymer | 65 | 19863.7 | 1 | Streptomyces coelicolor A3(2) | ||
10 | P | DNA (65-MER) | polymer | 65 | 20086.8 | 1 | Streptomyces coelicolor A3(2) | ||
11 | D | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
12 | D | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
D: 1 - 1299 (UniProt: Q8CJT1)
F: 1 - 511 (UniProt: P18183)
I, J: 1 - 270 (UniProt: P25941)
PDB | External Database | Details |
---|---|---|
His 1300 | - | expression tag |
His 1301 | - | expression tag |
His 1302 | - | expression tag |
His 1303 | - | expression tag |
His 1304 | - | expression tag |
His 1305 | - | expression tag |
His 1306 | - | expression tag |
His 1307 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 13 |
Total formula weight | 587578.1 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 155.1 | |
All* | Total formula weight | 587733.2 |