8HCX

Cryo-EM structure of Endothelin1-bound ETBR-Gq complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(q) subunit alpha-1	polymer	246	28084.8	1		Homo sapiens
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	377	41055.9	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (human)	Transducin beta chain 1
3	C	Endothelin receptor type B,Oplophorus-luciferin 2-monooxygenase catalytic subunit chimera	polymer	603	67882.3	1	UniProt (P24530) UniProt (Q9GV45) Pfam (PF00001) In PDB	Homo sapiens (human)	ET-B,ET-BR,Endothelin receptor non-selective type,19kOLase
4	D	Endothelin-1	polymer	21	2498.0	1	UniProt (P05305) Pfam (PF00322) In PDB	Homo sapiens (human)	Preproendothelin-1,PPET1
5	E	scFv16	polymer	285	30363.0	1		Mus musculus
6	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (human)	G gamma-I

Sequence modifications

B: 7 - 345 (UniProt: P62873)

PDB	External Database	Details
Met -5	-	initiating methionine
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
Gly 2	-	expression tag
Ser 3	-	expression tag
Leu 4	-	expression tag
Leu 5	-	expression tag
Gln 6	-	expression tag
Gly 346	-	expression tag
Ser 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Gly 351	-	expression tag
Gly 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Ser 359	-	expression tag
Gly 360	-	expression tag
Val 361	-	expression tag
Ser 362	-	expression tag
Gly 363	-	expression tag
Trp 364	-	expression tag
Arg 365	-	expression tag
Leu 366	-	expression tag
Phe 367	-	expression tag
Lys 368	-	expression tag
Lys 369	-	expression tag
Ile 370	-	expression tag
Ser 371	-	expression tag

C: 27 - 424 (UniProt: P24530)

PDB	External Database	Details
Met -20	-	initiating methionine
Asp -19	-	expression tag
Ser -18	-	expression tag
Lys -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gln -13	-	expression tag
Lys -12	-	expression tag
Gly -11	-	expression tag
Ser -10	-	expression tag
Arg -9	-	expression tag
Leu -8	-	expression tag
Leu -7	-	expression tag
Leu -6	-	expression tag
Leu -5	-	expression tag
Leu -4	-	expression tag
Val -3	-	expression tag
Val -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Leu 1	-	expression tag
Leu 2	-	expression tag
Leu 3	-	expression tag
Cys 4	-	expression tag
Gln 5	-	expression tag
Gly 6	-	expression tag
Val 7	-	expression tag
Val 8	-	expression tag
Ser 9	-	expression tag
Asp 10	-	expression tag
Tyr 11	-	expression tag
Lys 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Asp 16	-	expression tag
Val 17	-	expression tag
Asp 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
His 22	-	expression tag
His 23	-	expression tag
His 24	-	expression tag
His 25	-	expression tag
His 26	-	expression tag

C: 425 - 582 (UniProt: Q9GV45)

PDB	External Database	Details
Val 425	Thr 27	conflict
Glu 429	Ala 31	conflict
Glu 436	Gln 38	conflict
Ala 440	Gly 42	conflict
Leu 443	Gln 45	conflict
Val 452	Leu 54	conflict
Leu 456	Phe 58	conflict
Asn 458	Ala 60	conflict
Ala 460	Gly 62	conflict
Arg 468	Lys 70	conflict
Ile 469	Val 71	conflict
Arg 471	Leu 73	conflict
Ala 476	Gly 78	conflict
Ile 479	Ala 81	conflict
Ala 492	Gly 94	conflict
Asp 493	Phe 95	conflict
Ala 496	Gly 98	conflict
Gln 497	Leu 99	conflict
Glu 500	Met 102	conflict
Val 501	Ile 103	conflict
Val 515	Ile 117	conflict
Pro 518	His 120	conflict
Leu 532	Ile 134	conflict
Asn 533	Asp 135	conflict
Glu 540	Pro 142	conflict
Lys 549	Gln 151	conflict
Ile 563	Tyr 165	conflict
Thr 569	Asn 171	conflict
Met 574	Leu 176	conflict
Ser 582	Gly 184	conflict

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	177745.2
All*	Total formula weight	177745.2

*Water molecules are not included.