8H6L
Cryo-EM structure of human exon-defined spliceosome in the early B state.
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | pre-mRNA | polymer | 144 | 45961.0 | 1 | Homo sapiens (human) | ||
| 2 | 5A | U5 snRNA | polymer | 117 | 37254.9 | 1 | GenBank (20330981) | Homo sapiens (human) | |
| 3 | 5B | Pre-mRNA-processing-splicing factor 8 | polymer | 2335 | 273974.3 | 1 | UniProt (Q6P2Q9) Pfam (PF08082) Pfam (PF08083) Pfam (PF10598) Pfam (PF10597) Pfam (PF10596) Pfam (PF12134) Pfam (PF01398) Pfam (PF08084) In PDB | Homo sapiens (human) | 220 kDa U5 snRNP-specific protein,PRP8 homolog,Splicing factor Prp8,p220 |
| 4 | 5C | 116 kDa U5 small nuclear ribonucleoprotein component | polymer | 972 | 109560.6 | 1 | UniProt (Q15029) Pfam (PF16004) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679) In PDB | Homo sapiens (human) | Elongation factor Tu GTP-binding domain-containing protein 2,SNU114 homolog,hSNU114,U5 snRNP-specific protein,116 kDa,U5-116 kDa |
| 5 | 5D | U5 small nuclear ribonucleoprotein 200 kDa helicase | polymer | 2136 | 244823.4 | 1 | UniProt (O75643) Pfam (PF21188) Pfam (PF18149) Pfam (PF00270) Pfam (PF00271) Pfam (PF02889) In PDB | Homo sapiens (human) | Activating signal cointegrator 1 complex subunit 3-like 1,BRR2 homolog,U5 snRNP-specific 200 kDa protein,U5-200KD |
| 6 | 5E | U5 small nuclear ribonucleoprotein 40 kDa protein | polymer | 357 | 39359.5 | 1 | UniProt (Q96DI7) Pfam (PF00400) In PDB | Homo sapiens (human) | U5 snRNP 40 kDa protein,U5-40K,38 kDa-splicing factor,Prp8-binding protein,hPRP8BP,U5 snRNP-specific 40 kDa protein,WD repeat-containing protein 57 |
| 7 | 5a, 4a, 2a | Isoform SM-B of Small nuclear ribonucleoprotein-associated proteins B and B' | polymer | 231 | 23686.0 | 3 | UniProt (P14678) Pfam (PF01423) In PDB | Homo sapiens (human) | snRNP-B,Sm protein B/B',Sm-B/B',SmB/B' |
| 8 | 5b, 4b, 2b | Small nuclear ribonucleoprotein Sm D1 | polymer | 119 | 13310.7 | 3 | UniProt (P62314) Pfam (PF01423) In PDB | Homo sapiens (human) | Sm-D1,Sm-D autoantigen,snRNP core protein D1 |
| 9 | 5c, 4c, 2c | Small nuclear ribonucleoprotein Sm D2 | polymer | 118 | 13551.9 | 3 | UniProt (P62316) Pfam (PF01423) In PDB | Homo sapiens (human) | Sm-D2,snRNP core protein D2 |
| 10 | 5d, 4d, 2d | Small nuclear ribonucleoprotein F | polymer | 86 | 9734.2 | 3 | UniProt (P62306) Pfam (PF01423) In PDB | Homo sapiens (human) | snRNP-F,Sm protein F,Sm-F,SmF |
| 11 | 5e, 4e, 2e | Small nuclear ribonucleoprotein E | polymer | 92 | 10817.6 | 3 | UniProt (P62304) Pfam (PF01423) In PDB | Homo sapiens (human) | snRNP-E,Sm protein E,Sm-E,SmE |
| 12 | 5f, 4f, 2f | Small nuclear ribonucleoprotein G | polymer | 76 | 8508.1 | 3 | UniProt (P62308) Pfam (PF01423) In PDB | Homo sapiens (human) | snRNP-G,Sm protein G,Sm-G,SmG |
| 13 | 5g, 4g, 2g | Small nuclear ribonucleoprotein Sm D3 | polymer | 126 | 13940.3 | 3 | UniProt (P62318) Pfam (PF01423) In PDB | Homo sapiens (human) | Sm-D3,snRNP core protein D3 |
| 14 | 6A | U6 snRNA | polymer | 107 | 34404.4 | 1 | GenBank (1621863533) | Homo sapiens (human) | |
| 15 | 6a | U6 snRNA-associated Sm-like protein LSm2 | polymer | 95 | 10847.5 | 1 | UniProt (Q9Y333) Pfam (PF01423) In PDB | Homo sapiens (human) | Protein G7b,Small nuclear ribonuclear protein D homolog,snRNP core Sm-like protein Sm-x5 |
| 16 | 6b | U6 snRNA-associated Sm-like protein LSm3 | polymer | 102 | 11859.4 | 1 | UniProt (P62310) Pfam (PF01423) In PDB | Homo sapiens (human) | |
| 17 | 6c | U6 snRNA-associated Sm-like protein LSm4 | polymer | 139 | 15375.8 | 1 | UniProt (Q9Y4Z0) Pfam (PF01423) In PDB | Homo sapiens (human) | Glycine-rich protein,GRP |
| 18 | 6d | U6 snRNA-associated Sm-like protein LSm5 | polymer | 91 | 9945.4 | 1 | UniProt (Q9Y4Y9) Pfam (PF01423) In PDB | Homo sapiens (human) | |
| 19 | 6e | U6 snRNA-associated Sm-like protein LSm6 | polymer | 80 | 9139.6 | 1 | UniProt (P62312) Pfam (PF01423) In PDB | Homo sapiens (human) | |
| 20 | 6f | U6 snRNA-associated Sm-like protein LSm7 | polymer | 103 | 11617.4 | 1 | UniProt (Q9UK45) Pfam (PF01423) In PDB | Homo sapiens (human) | |
| 21 | 6g | U6 snRNA-associated Sm-like protein LSm8 | polymer | 96 | 10410.6 | 1 | UniProt (O95777) Pfam (PF01423) In PDB | Homo sapiens (human) | |
| 22 | 4A | U4 snRNA | polymer | 145 | 46650.6 | 1 | Homo sapiens (human) | ||
| 23 | 4B | U4/U6 small nuclear ribonucleoprotein Prp3 | polymer | 683 | 77669.2 | 1 | UniProt (O43395) Pfam (PF01480) Pfam (PF08572) Pfam (PF06544) In PDB | Homo sapiens (human) | Pre-mRNA-splicing factor 3,hPrp3,U4/U6 snRNP 90 kDa protein |
| 24 | 4C | U4/U6 small nuclear ribonucleoprotein Prp4 | polymer | 522 | 58536.1 | 1 | UniProt (O43172) Pfam (PF08799) Pfam (PF00400) In PDB | Homo sapiens (human) | PRP4 homolog,hPrp4,U4/U6 snRNP 60 kDa protein,WD splicing factor Prp4 |
| 25 | 4D | U4/U6 small nuclear ribonucleoprotein Prp31 | polymer | 499 | 55529.0 | 1 | UniProt (Q8WWY3) Pfam (PF01798) Pfam (PF09785) In PDB | Homo sapiens (human) | Pre-mRNA-processing factor 31,Serologically defined breast cancer antigen NY-BR-99,U4/U6 snRNP 61 kDa protein,Protein 61K,hPrp31 |
| 26 | 4E | NHP2-like protein 1 | polymer | 128 | 14191.5 | 1 | UniProt (P55769) Pfam (PF01248) In PDB | Homo sapiens (human) | High mobility group-like nuclear protein 2 homolog 1,OTK27,SNU13 homolog,hSNU13,U4/U6.U5 small nuclear ribonucleoprotein SNU13,U4/U6.U5 tri-snRNP 15.5 kDa protein |
| 27 | 4F | Thioredoxin-like protein 4A | polymer | 142 | 16807.3 | 1 | UniProt (P83876) Pfam (PF02966) In PDB | Homo sapiens (human) | DIM1 protein homolog,Spliceosomal U5 snRNP-specific 15 kDa protein,Thioredoxin-like U5 snRNP protein U5-15kD |
| 28 | 4G | Pre-mRNA-processing factor 6 | polymer | 941 | 107092.2 | 1 | UniProt (O94906) Pfam (PF06424) Pfam (PF14559) Pfam (PF13181) In PDB | Homo sapiens (human) | Androgen receptor N-terminal domain-transactivating protein 1,ANT-1,PRP6 homolog,U5 snRNP-associated 102 kDa protein,U5-102 kDa protein |
| 29 | 4H | Peptidyl-prolyl cis-trans isomerase H | polymer | 177 | 19230.1 | 1 | UniProt (O43447) Pfam (PF00160) In PDB | Homo sapiens (human) | PPIase H,Rotamase H,Small nuclear ribonucleoprotein particle-specific cyclophilin H,CypH,U-snRNP-associated cyclophilin SnuCyp-20,USA-CYP |
| 30 | 4I | WW domain-binding protein 4 | polymer | 376 | 42575.8 | 1 | UniProt (O75554) Pfam (PF06220) Pfam (PF00397) In PDB | Homo sapiens (human) | WBP-4,Formin-binding protein 21,WW domain-containing-binding protein 4 |
| 31 | 4J | U4/U6.U5 tri-snRNP-associated protein 1 | polymer | 800 | 90414.1 | 1 | UniProt (O43290) Pfam (PF19252) Pfam (PF03343) In PDB | Homo sapiens (human) | SNU66 homolog,hSnu66,Squamous cell carcinoma antigen recognized by T-cells 1,SART-1,hSART-1,U4/U6.U5 tri-snRNP-associated 110 kDa protein |
| 32 | 4Z | WD40 repeat-containing protein SMU1 | polymer | 513 | 57616.7 | 1 | UniProt (Q2TAY7) Pfam (PF17814) Pfam (PF00400) In PDB | Homo sapiens (human) | Smu-1 suppressor of mec-8 and unc-52 protein homolog |
| 33 | 2A | U2 snRNA | polymer | 188 | 60186.4 | 1 | Homo sapiens (human) | ||
| 34 | 2B | U2 small nuclear ribonucleoprotein A' | polymer | 255 | 28456.6 | 1 | UniProt (P09661) Pfam (PF14580) In PDB | Homo sapiens (human) | U2 snRNP A' |
| 35 | 2C | U2 small nuclear ribonucleoprotein B'' | polymer | 225 | 25524.4 | 1 | UniProt (P08579) Pfam (PF00076) In PDB | Homo sapiens (human) | U2 snRNP B'' |
| 36 | 2D | Splicing factor 3A subunit 1 | polymer | 793 | 88991.1 | 1 | UniProt (Q15459) Pfam (PF01805) Pfam (PF12230) Pfam (PF00240) In PDB | Homo sapiens (human) | SF3a120,Spliceosome-associated protein 114,SAP 114 |
| 37 | 2E | Splicing factor 3A subunit 2 | polymer | 464 | 49327.4 | 1 | UniProt (Q15428) Pfam (PF12874) Pfam (PF16835) In PDB | Homo sapiens (human) | SF3a66,Spliceosome-associated protein 62,SAP 62 |
| 38 | 2F | Splicing factor 3A subunit 3 | polymer | 501 | 58934.8 | 1 | UniProt (Q12874) Pfam (PF12108) Pfam (PF16837) Pfam (PF13297) Pfam (PF11931) In PDB | Homo sapiens (human) | SF3a60,Spliceosome-associated protein 61,SAP 61 |
| 39 | 2G | Splicing factor 3B subunit 1 | polymer | 1304 | 146024.9 | 1 | UniProt (O75533) Pfam (PF08920) In PDB | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 155 kDa subunit,SF3b155,Spliceosome-associated protein 155,SAP 155 |
| 40 | 2H | Splicing factor 3B subunit 2 | polymer | 895 | 100377.8 | 1 | UniProt (Q13435) Pfam (PF02037) Pfam (PF04037) Pfam (PF04046) In PDB | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 145 kDa subunit,SF3b145,Spliceosome-associated protein 145,SAP 145 |
| 41 | 2I | Splicing factor 3B subunit 3 | polymer | 1217 | 135718.8 | 1 | UniProt (Q15393) Pfam (PF10433) Pfam (PF03178) In PDB | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 130 kDa subunit,SF3b130,STAF130,Spliceosome-associated protein 130,SAP 130 |
| 42 | 2J | Splicing factor 3B subunit 4 | polymer | 424 | 44436.6 | 1 | UniProt (Q15427) Pfam (PF00076) In PDB | Homo sapiens (human) | Pre-mRNA-splicing factor SF3b 49 kDa subunit,Spliceosome-associated protein 49,SAP 49 |
| 43 | 2K | Splicing factor 3B subunit 6 | polymer | 125 | 14606.9 | 1 | UniProt (Q9Y3B4) Pfam (PF00076) In PDB | Homo sapiens (human) | Pre-mRNA branch site protein p14,SF3b 14 kDa subunit,SF3B14a,Spliceosome-associated protein,14-kDa,Splicing factor 3b,subunit 6,14kDa |
| 44 | 2L | PHD finger-like domain-containing protein 5A | polymer | 110 | 12427.5 | 1 | UniProt (Q7RTV0) Pfam (PF03660) In PDB | Homo sapiens (human) | PHD finger-like domain protein 5A,Splicing factor 3B-associated 14 kDa protein,SF3b14b |
| 45 | 2M | Splicing factor 3B subunit 5 | polymer | 86 | 10149.4 | 1 | UniProt (Q9BWJ5) Pfam (PF07189) In PDB | Homo sapiens (human) | SF3b5,Pre-mRNA-splicing factor SF3b 10 kDa subunit |
| 46 | 5B | INOSITOL HEXAKISPHOSPHATE | non-polymer | 660.0 | 1 | Chemie (IHP) | |||
| 47 | 5C | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 48 | 5C | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 49 | 4I | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 59 |
| Total formula weight | 2506655.3 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1272.9 | |
| All* | Total formula weight | 2507928.2 |
