8H53

Human asparaginyl-tRNA synthetase in complex with asparagine-AMP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Asparagine--tRNA ligase, cytoplasmic	polymer	472	54007.5	4	UniProt (O43776) Pfam (PF01336) Pfam (PF00152)	Homo sapiens (human)	Asparaginyl-tRNA synthetase,AsnRS,Asparaginyl-tRNA synthetase 1
2	BA, E, LA, R (C, A, D, B)	4-AMINO-1,4-DIOXOBUTAN-2-AMINIUM ADENOSINE-5'-MONOPHOSPHATE	non-polymer		462.3	4	Chemie (4AD)
3	CA, F, MA, S (C, A, D, B)	IMIDODIPHOSPHORIC ACID	non-polymer		177.0	4	Chemie (2PN)
4	DA, EA, FA, G, GA... (C, A, D, B)	GLYCEROL	non-polymer		92.1	26	Chemie (GOL)
5	AA, JA, KA, P, Q... (B, C, A, D)	MAGNESIUM ION	non-polymer		24.3	8	Chemie (MG)
6	UA, VA, WA, XA (A, B, C, D)	water	water		18.0	746	Chemie (HOH)

Sequence modifications

A, B, C, D: 98 - 548 (UniProt: O43776)

PDB	External Database	Details
Met 77	-	initiating methionine
Gly 78	-	expression tag
Ser 79	-	expression tag
Ser 80	-	expression tag
His 81	-	expression tag
His 82	-	expression tag
His 83	-	expression tag
His 84	-	expression tag
His 85	-	expression tag
His 86	-	expression tag
Ser 87	-	expression tag
Ser 88	-	expression tag
Gly 89	-	expression tag
Leu 90	-	expression tag
Val 91	-	expression tag
Pro 92	-	expression tag
Arg 93	-	expression tag
Gly 94	-	expression tag
Ser 95	-	expression tag
His 96	-	expression tag
Met 97	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	216030.1
Non-Polymers*	Number of molecules	42
	Total formula weight	5146.2
All*	Total formula weight	221176.3

*Water molecules are not included.