8H53

Human asparaginyl-tRNA synthetase in complex with asparagine-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	115.423, 125.842, 161.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.880 - 2.160
R-factor	0.205
Rwork	0.203
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xix
RMSD bond length	0.003
RMSD bond angle	0.573
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.160	2.160
Number of reflections	126122	6260
<I/σ(I)>	12.3	2.1
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)	0.993	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	40 mM potassium phosphate, 20% (v/v) glycerol, 16% (w/v) polyethylene glycol 8,000