8H3V
Cryo-EM structure of the full transcription activation complex NtcA-NtcB-TAC
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (1) | DNA (125-MER) | polymer | 125 | 38723.9 | 1 | Anabaena | ||
| 2 | B (2) | DNA (125-MER) | polymer | 125 | 38571.7 | 1 | Anabaena | ||
| 3 | C (A) | DNA-directed RNA polymerase subunit beta | polymer | 1132 | 126781.5 | 1 | UniProt (P22703) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) | Anabaena | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 4 | D (B) | DNA-directed RNA polymerase subunit beta' | polymer | 1350 | 147137.5 | 1 | UniProt (P22705) Pfam (PF04998) Pfam (PF04983) Pfam (PF05000) | Anabaena | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 5 | E, F (C, D) | DNA-directed RNA polymerase subunit alpha | polymer | 236 | 26166.6 | 2 | UniProt (Q8YPK3) Pfam (PF01193) Pfam (PF01000) | Anabaena | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 6 | G (E) | DNA-directed RNA polymerase subunit gamma | polymer | 625 | 70653.1 | 1 | UniProt (P22704) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) | Anabaena | RNAP subunit gamma,RNA polymerase subunit gamma,Transcriptase subunit gamma |
| 7 | H (F) | DNA-directed RNA polymerase subunit omega | polymer | 78 | 9143.5 | 1 | UniProt (Q8YNB9) Pfam (PF01192) | Anabaena | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 8 | I (G) | RNA polymerase sigma factor SigA | polymer | 390 | 45717.0 | 1 | UniProt (P26683) Pfam (PF00140) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) | Anabaena | Sigma-A |
| 9 | J, K, L, M (S, T, U, V) | NtcB | polymer | 312 | 34941.4 | 4 | UniProt (Q9L3R4) Pfam (PF00126) Pfam (PF03466) | Anabaena | |
| 10 | N, O (X, Y) | NtcA | polymer | 223 | 24951.3 | 2 | UniProt (P0A4U6) Pfam (PF00027) Pfam (PF13545) | Anabaena | DNA-binding protein VF1,Nitrogen-responsive regulatory protein |
Sequence modifications
A: 2 - 1131 (UniProt: P22703)
C, D: 2 - 235 (UniProt: Q8YPK3)
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Val 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Val 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 15 |
| Total formula weight | 718729.4 | |
| All* | Total formula weight | 718729.4 |
