8GNK
CryoEM structure of cytosol-facing, substrate-bound ratGAT1
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Sodium- and chloride-dependent GABA transporter 1 | polymer | 562 | 63200.7 | 1 | UniProt (P23978) Pfam (PF00209) In PDB | Rattus norvegicus (Norway rat) | GAT-1,Solute carrier family 6 member 1 |
2 | L | Fragment antigen binding light chain | polymer | 214 | 23306.6 | 1 | Mus musculus (house mouse) | ||
3 | H | fragment antigen binding 9D5 heavy chain | polymer | 219 | 23619.4 | 1 | Mus musculus | ||
4 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
6 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
7 | A | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 1 | Chemie (ABU) | |||
8 | A | CHOLESTEROL | non-polymer | 386.7 | 6 | Chemie (CLR) | |||
9 | A | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 1 | Chemie (PTY) | |||
10 | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A: 39 - 599 (UniProt: P23978)
PDB | External Database | Details |
---|---|---|
Met 38 | - | initiating methionine |
Tyr 312 | Phe 312 | engineered mutation |
Ser 481 | Tyr 481 | engineered mutation |
Glu 482 | Asp 482 | engineered mutation |
Asp 483 | Asn 483 | engineered mutation |
Arg 485 | Gln 485 | engineered mutation |
Asp 486 | Glu 486 | engineered mutation |
Ile 488 | Val 488 | engineered mutation |
Phe 490 | Ser 490 | engineered mutation |
Pro 491 | Arg 491 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 110126.7 | |
Non-Polymers* | Number of molecules | 12 |
Total formula weight | 3657.9 | |
All* | Total formula weight | 113784.6 |