8GNK
CryoEM structure of cytosol-facing, substrate-bound ratGAT1
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Sodium- and chloride-dependent GABA transporter 1 | polymer | 562 | 63200.7 | 1 | UniProt (P23978) Pfam (PF00209) | Rattus norvegicus (Norway rat) | GAT-1,Solute carrier family 6 member 1 |
| 2 | B (L) | Fragment antigen binding light chain | polymer | 214 | 23306.6 | 1 | Mus musculus (house mouse) | ||
| 3 | C (H) | fragment antigen binding 9D5 heavy chain | polymer | 219 | 23619.4 | 1 | Mus musculus | ||
| 4 | D, E (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) | |||
| 5 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 6 | G (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 7 | H (A) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 1 | Chemie (ABU) | |||
| 8 | I, J, K, L, M... (A) | CHOLESTEROL | non-polymer | 386.7 | 6 | Chemie (CLR) | |||
| 9 | O (A) | PHOSPHATIDYLETHANOLAMINE | non-polymer | 734.0 | 1 | Chemie (PTY) | |||
| 10 | P (A) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A: 39 - 599 (UniProt: P23978)
| PDB | External Database | Details |
|---|---|---|
| Met 38 | - | initiating methionine |
| Tyr 312 | Phe 312 | engineered mutation |
| Ser 481 | Tyr 481 | engineered mutation |
| Glu 482 | Asp 482 | engineered mutation |
| Asp 483 | Asn 483 | engineered mutation |
| Arg 485 | Gln 485 | engineered mutation |
| Asp 486 | Glu 486 | engineered mutation |
| Ile 488 | Val 488 | engineered mutation |
| Phe 490 | Ser 490 | engineered mutation |
| Pro 491 | Arg 491 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 110126.7 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 3657.9 | |
| All* | Total formula weight | 113784.6 |
