8GKM
A1 Leu graft + Leu: Adenylation domain 1 core construct from teicoplanin biosynthesis, leucine selection pocket graft; leucine bound
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Non-ribosomal peptide synthetase | polymer | 399 | 42145.8 | 2 | UniProt (Q70AZ9) Pfam (PF00501) UniProt (by SIFTS) (A0A561VI36) In PDB | Actinoplanes teichomyceticus | |
2 | C, D | MbtH-like short polypeptide | polymer | 69 | 7636.3 | 2 | UniProt (Q70AZ5) Pfam (PF03621) UniProt (by SIFTS) (Q6ZZJ2) In PDB | Actinoplanes teichomyceticus | |
3 | A, B | LEUCINE | non-polymer | 131.2 | 2 | Chemie (LEU) | |||
4 | A | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
5 | A | ACETIC ACID | non-polymer | 60.1 | 1 | Chemie (ACY) | |||
6 | A | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
7 | water | water | 18.0 | 358 | Chemie (HOH) |
Sequence modifications
A, B: 9 - 398 (UniProt: Q70AZ9)
PDB | External Database | Details |
---|---|---|
Met 8 | - | initiating methionine |
Tyr 237 | His 237 | engineered mutation |
Met 287 | Leu 287 | engineered mutation |
Met 295 | Leu 295 | engineered mutation |
Gly 399 | - | expression tag |
Ser 400 | - | expression tag |
His 401 | - | expression tag |
His 402 | - | expression tag |
His 403 | - | expression tag |
His 404 | - | expression tag |
His 405 | - | expression tag |
His 406 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 99564.2 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 451.5 | |
All* | Total formula weight | 100015.7 |