8GBJ

Cryo-EM structure of a human BCDX2/ssDNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B	DNA repair protein RAD51 homolog 2	polymer	356	39125.9	1	UniProt (O15315) Pfam (PF08423) In PDB	Homo sapiens (human)	R51H2,RAD51 homolog B,Rad51B,RAD51-like protein 1
2	C	DNA repair protein RAD51 homolog 3	polymer	376	42244.6	1	UniProt (O43502) Pfam (PF08423) In PDB	Homo sapiens (human)	R51H3,RAD51 homolog C,RAD51-like protein 2
3	D	DNA repair protein RAD51 homolog 4	polymer	328	35084.3	1	UniProt (O75771) Pfam (PF21794) Pfam (PF08423) In PDB	Homo sapiens (human)	R51H3,RAD51 homolog D,RAD51-like protein 3,TRAD
4	X	DNA repair protein XRCC2	polymer	288	32992.5	1	UniProt (O43543) Pfam (PF08423) In PDB	Homo sapiens (human)	X-ray repair cross-complementing protein 2
5	G	DNA (5'-D(P*CP*CP*CP*CP*CP*C)-3')	polymer	30	8630.5	1		synthetic construct
6	C, D, X	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	3	Chemie (ANP)

Sequence modifications

B: 1 - 350 (UniProt: O15315)

PDB	External Database	Details
His 351	-	expression tag
His 352	-	expression tag
His 353	-	expression tag
His 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag

X: 1 - 280 (UniProt: O43543)

PDB	External Database	Details
Asp 281	-	expression tag
Tyr 282	-	expression tag
Lys 283	-	expression tag
Asp 284	-	expression tag
Asp 285	-	expression tag
Asp 286	-	expression tag
Asp 287	-	expression tag
Lys 288	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	158077.7
Non-Polymers*	Number of molecules	3
	Total formula weight	1518.6
All*	Total formula weight	159596.3

*Water molecules are not included.