8G8W

Molecular mechanism of nucleotide inhibition of human uncoupling protein 1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Mitochondrial brown fat uncoupling protein 1	polymer	310	33339.6	1	UniProt (P25874) Pfam (PF00153)	Homo sapiens (human)	UCP 1,Solute carrier family 25 member 7,Thermogenin
2	B (B)	Pro-macrobody 71, Maltose/maltodextrin-binding periplasmic protein chimera	polymer	490	53453.9	1	UniProt (P0AEX9) Pfam (PF01547)	synthetic construct	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
3	C (C)	Pro-Macrobody 65, Maltose/maltodextrin-binding periplasmic protein chimera	polymer	494	54052.8	1	UniProt (P0AEX9) Pfam (PF01547)	synthetic construct	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP
4	D (D)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB GlyTouCan (G07411ON)
5	E (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
6	F, G, H (A)	CARDIOLIPIN	non-polymer		1464.0	3	Chemie (CDL)

Sequence modifications

A: 2 - 307 (UniProt: P25874)

PDB	External Database	Details
Thr -2	-	expression tag
Ser -1	-	expression tag
Glu 0	-	expression tag
Asp 1	-	expression tag

B: 1 - 127 (PDB: 8G8W)
B: 128 - 487 (UniProt: P0AEX9)

PDB	External Database	Details
Pro 488	-	expression tag
Gly 489	-	expression tag
Ser 490	-	expression tag

C: 1 - 131 (PDB: 8G8W)
C: 132 - 491 (UniProt: P0AEX9)

PDB	External Database	Details
Pro 492	-	expression tag
Gly 493	-	expression tag
Ser 494	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	140846.4
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	4
	Total formula weight	4915.3
All*	Total formula weight	146104.0

*Water molecules are not included.