8G46

Cryo-EM structure of DDB1deltaB-DDA1-DCAF16-BRD4(BD2)-MMH2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA damage-binding protein 1	polymer	864	96425.6	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178) In PDB	Homo sapiens (human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
2	B	DDB1- and CUL4-associated factor 16	polymer	220	24547.7	1	UniProt (Q9NXF7) Pfam (PF15349) In PDB	Homo sapiens (human)
3	C	Bromodomain-containing protein 4	polymer	129	14899.1	1	UniProt (O60885) Pfam (PF00439) In PDB	Homo sapiens (human)	Protein HUNK1
4	E	DET1- and DDB1-associated protein 1	polymer	106	12183.6	1	UniProt (Q9BW61) Pfam (PF10172) In PDB	Homo sapiens (human)	Placenta cross-immune reaction antigen 1,PCIA-1
5	B	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	B	tert-butyl [(6S,10P)-4-{4-[(ethanesulfonyl)amino]phenyl}-2,3,9-trimethyl-6H-thieno[3,2-f][1,2,4]triazolo[4,3-a][1,4]diazepin-6-yl]acetate	non-polymer		529.7	1	Chemie (YK3)
7		water	water		18.0	110	Chemie (HOH)

Sequence modifications

A: 1 - 699 (UniProt: Q16531)

PDB	External Database	Details
Met -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Ala -15	-	expression tag
His -14	-	expression tag
Ile -13	-	expression tag
Val -12	-	expression tag
Met -11	-	expression tag
Val -10	-	expression tag
Asp -9	-	expression tag
Ala -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Pro -5	-	expression tag
Thr -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

A: 706 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Gly 700	-	linker
Asn 701	-	linker
Gly 702	-	linker
Asn 703	-	linker
Ser 704	-	linker
Gly 705	-	linker

B: 1 - 216 (UniProt: Q9NXF7)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

C: 333 - 460 (UniProt: O60885)

PDB	External Database	Details
Gly 332	-	expression tag

E: 1 - 102 (UniProt: Q9BW61)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	148056.0
Non-Polymers*	Number of molecules	2
	Total formula weight	595.1
All*	Total formula weight	148651.1

*Water molecules are not included.