8G04

Structure of signaling thrombopoietin-MPL receptor complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Thrombopoietin	polymer	171	18381.3	1	UniProt (P40225) Pfam (PF00758) In PDB	Homo sapiens (human)	C-mpl ligand,ML,Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor,MGDF,Myeloproliferative leukemia virus oncogene ligand
2	B, C	Thrombopoietin receptor	polymer	636	71493.4	2	UniProt (P40238) Pfam (PF09067) In PDB	Homo sapiens (human)	TPO-R,Myeloproliferative leukemia protein,Proto-oncogene c-Mpl
3	B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)
4	B, C	alpha-D-mannopyranose	non-polymer		180.2	6	Chemie (MAN)

Sequence modifications

A: 22 - 184 (UniProt: P40225)

PDB	External Database	Details
Asp 14	-	expression tag
Tyr 15	-	expression tag
Lys 16	-	expression tag
Asp 17	-	expression tag
Asp 18	-	expression tag
Asp 19	-	expression tag
Asp 20	-	expression tag
Gly 21	-	expression tag

B, C: 26 - 635 (UniProt: P40238)

PDB	External Database	Details
Ala 636	-	expression tag
Ala 637	-	expression tag
Ala 638	-	expression tag
Gly 639	-	expression tag
Ala 640	-	expression tag
Ala 641	-	expression tag
Glu 642	-	expression tag
Asp 643	-	expression tag
Gln 644	-	expression tag
Val 645	-	expression tag
Asp 646	-	expression tag
Pro 647	-	expression tag
Arg 648	-	expression tag
Leu 649	-	expression tag
Ile 650	-	expression tag
Asp 651	-	expression tag
Gly 652	-	expression tag
Lys 653	-	expression tag
His 654	-	expression tag
His 655	-	expression tag
His 656	-	expression tag
His 657	-	expression tag
His 658	-	expression tag
His 659	-	expression tag
His 660	-	expression tag
His 661	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	161368.2
Non-Polymers*	Number of molecules	12
	Total formula weight	2408.2
All*	Total formula weight	163776.4

*Water molecules are not included.