8EAZ

HOIL-1/E2-Ub/Ub transthiolation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	RanBP-type and C3HC4-type zinc finger-containing protein 1	polymer	280	31770.0	2	UniProt (Q9BYM8) Pfam (PF13445) In PDB	Homo sapiens (human)	HBV-associated factor 4,Heme-oxidized IRP2 ubiquitin ligase 1,HOIL-1,Hepatitis B virus X-associated protein 4,RING finger protein 54,RING-type E3 ubiquitin transferase HOIL-1,Ubiquitin-conjugating enzyme 7-interacting protein 3
2	C, D	Ubiquitin-conjugating enzyme E2 L3	polymer	157	18126.8	2	UniProt (P68036) Pfam (PF00179) In PDB	Homo sapiens (human)	E2 ubiquitin-conjugating enzyme L3,L-UBC,UbcH7,Ubiquitin carrier protein L3,Ubiquitin-conjugating enzyme E2-F1,Ubiquitin-protein ligase L3
3	E, F, G, H	Ubiquitin	polymer	76	8576.8	4	UniProt (P0CG48) Pfam (PF00240) In PDB	Homo sapiens (human)
4	A, B	ZINC ION	non-polymer		65.4	14	Chemie (ZN)
5		water	water		18.0	8	Chemie (HOH)

Sequence modifications

A, B: 232 - 510 (UniProt: Q9BYM8)

PDB	External Database	Details
Gly 231	-	expression tag
Ala 460	Cys 460	engineered mutation

C, D: 1 - 154 (UniProt: P68036)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
Gly 0	-	expression tag
Lys 86	Cys 86	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	134101.0
Non-Polymers*	Number of molecules	14
	Total formula weight	915.7
All*	Total formula weight	135016.8

*Water molecules are not included.