Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8EAZ

HOIL-1/E2-Ub/Ub transthiolation complex

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0000151	cellular_component	ubiquitin ligase complex
C	0000209	biological_process	protein polyubiquitination
C	0003713	molecular_function	transcription coactivator activity
C	0003723	molecular_function	RNA binding
C	0004842	molecular_function	ubiquitin-protein transferase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006355	biological_process	regulation of DNA-templated transcription
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0008283	biological_process	cell population proliferation
C	0016567	biological_process	protein ubiquitination
C	0016740	molecular_function	transferase activity
C	0019787	molecular_function	ubiquitin-like protein transferase activity
C	0019899	molecular_function	enzyme binding
C	0031398	biological_process	positive regulation of protein ubiquitination
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0032446	biological_process	protein modification by small protein conjugation
C	0036211	biological_process	protein modification process
C	0044770	biological_process	cell cycle phase transition
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0051443	biological_process	positive regulation of ubiquitin-protein transferase activity
C	0061631	molecular_function	ubiquitin conjugating enzyme activity
C	0070979	biological_process	protein K11-linked ubiquitination
C	0071383	biological_process	cellular response to steroid hormone stimulus
C	0071385	biological_process	cellular response to glucocorticoid stimulus
C	0097027	molecular_function	ubiquitin-protein transferase activator activity
C	0098793	cellular_component	presynapse
C	1903955	biological_process	positive regulation of protein targeting to mitochondrion
D	0000151	cellular_component	ubiquitin ligase complex
D	0000209	biological_process	protein polyubiquitination
D	0003713	molecular_function	transcription coactivator activity
D	0003723	molecular_function	RNA binding
D	0004842	molecular_function	ubiquitin-protein transferase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006355	biological_process	regulation of DNA-templated transcription
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0008283	biological_process	cell population proliferation
D	0016567	biological_process	protein ubiquitination
D	0016740	molecular_function	transferase activity
D	0019787	molecular_function	ubiquitin-like protein transferase activity
D	0019899	molecular_function	enzyme binding
D	0031398	biological_process	positive regulation of protein ubiquitination
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032446	biological_process	protein modification by small protein conjugation
D	0036211	biological_process	protein modification process
D	0044770	biological_process	cell cycle phase transition
D	0045893	biological_process	positive regulation of DNA-templated transcription
D	0051443	biological_process	positive regulation of ubiquitin-protein transferase activity
D	0061631	molecular_function	ubiquitin conjugating enzyme activity
D	0070979	biological_process	protein K11-linked ubiquitination
D	0071383	biological_process	cellular response to steroid hormone stimulus
D	0071385	biological_process	cellular response to glucocorticoid stimulus
D	0097027	molecular_function	ubiquitin-protein transferase activator activity
D	0098793	cellular_component	presynapse
D	1903955	biological_process	positive regulation of protein targeting to mitochondrion

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
E	LYS27-ASP52

site_id	PS00518
Number of Residues	10
Details	ZF_RING_1 Zinc finger RING-type signature. ClHtFCreCL

Chain	Residue	Details
A	CYS300-LEU309

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
E	ARG54
E	ARG72
F	ARG54
F	ARG72
G	ARG54
G	ARG72
H	ARG54
H	ARG72

site_id	SWS_FT_FI2
Number of Residues	4
Details	SITE: Essential for function

Chain	Residue	Details
E	HIS68
F	HIS68
G	HIS68
H	HIS68

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269\|PubMed:24660806, ECO:0000269\|PubMed:24751536, ECO:0000269\|PubMed:24784582, ECO:0000269\|PubMed:25527291

Chain	Residue	Details
E	SER65
F	SER65
G	SER65
H	SER65

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269\|PubMed:32330457

Chain	Residue	Details
E	THR66
F	THR66
G	THR66
H	THR66

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: ADP-ribosylglycine => ECO:0000269\|PubMed:28525742

Chain	Residue	Details
E	GLY76
F	GLY76
G	GLY76
H	GLY76
A	CYS305
A	CYS308
A	CYS323
A	CYS332
A	CYS371
A	CYS376
A	CYS391
A	CYS394
A	CYS399
A	CYS402
A	HIS406
A	CYS411
A	CYS447
A	CYS450
A	CYS465
A	CYS468
B	CYS282
B	CYS285
B	CYS300
B	HIS302
B	CYS305
B	CYS308
B	CYS323
B	CYS332
B	CYS371
B	CYS376
B	CYS391
B	CYS394
B	CYS399
B	CYS402
B	HIS406
B	CYS411
B	CYS447
B	CYS450
B	CYS465
B	CYS468

site_id	SWS_FT_FI6
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603

Chain	Residue	Details
E	LYS6
F	LYS6
G	LYS6
H	LYS6

site_id	SWS_FT_FI7
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)

Chain	Residue	Details
E	GLY76
F	GLY76
G	GLY76
H	GLY76

site_id	SWS_FT_FI8
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16443603, ECO:0000269\|PubMed:16543144

Chain	Residue	Details
E	LYS11
E	LYS48
F	LYS11
F	LYS48
G	LYS11
G	LYS48
H	LYS11
H	LYS48

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305\|PubMed:15466860

Chain	Residue	Details
E	LYS27
F	LYS27
G	LYS27
H	LYS27

site_id	SWS_FT_FI10
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:25752573, ECO:0000269\|PubMed:25752577, ECO:0000269\|PubMed:34239127

Chain	Residue	Details
E	LYS29
F	LYS29
G	LYS29
H	LYS29

site_id	SWS_FT_FI11
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:25752577

Chain	Residue	Details
E	LYS33
F	LYS33
G	LYS33
H	LYS33

site_id	SWS_FT_FI12
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269\|PubMed:16543144, ECO:0000269\|PubMed:18719106

Chain	Residue	Details
E	LYS63
F	LYS63
G	LYS63
H	LYS63

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	1
Details	M-CSA 438

Chain	Residue	Details
C	LYS86	nucleofuge

site_id	MCSA2
Number of Residues	1
Details	M-CSA 438

Chain	Residue	Details
D	LYS86	nucleofuge

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)