8DZZ

Cryo-EM structure of chi dynein bound to Lis1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Dynein heavy chain, cytoplasmic	polymer	2875	331524.0	2	UniProt (A0A8H4FAJ6) Pfam (PF08393) Pfam (PF18198) Pfam (PF12774) Pfam (PF21499) Pfam (PF12775) Pfam (PF17857) Pfam (PF12780) Pfam (PF12777) Pfam (PF12781) Pfam (PF03028) UniProt (by SIFTS) (P36022) In PDB	Saccharomyces cerevisiae (baker's yeast)	Dynein heavy chain,cytosolic
2	B, C, E, F	Nuclear distribution protein PAC1	polymer	495	57030.6	4	UniProt (P39946) Pfam (PF00400) In PDB	Saccharomyces cerevisiae (baker's yeast)	Lissencephaly-1 homolog,LIS-1,nudF homolog
3	A, D	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	6	Chemie (ATP)
4	A, D	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)

Sequence modifications

A, D: 1218 - 4092 (UniProt: A0A8H4FAJ6)

PDB	External Database	Details
Gly 1218	Asn 1218	conflict
Phe 1575	Leu 1575	conflict
Ser 1578	Phe 1578	conflict
Glu 1668	Gln 1668	conflict
Val 1777	Ile 1777	conflict
Val 1984	Ile 1984	conflict
Gln 2488	Glu 2488	engineered mutation
Val 2936	Ile 2936	conflict
Gln 3266	Arg 3266	conflict
Gly 3343	Ala 3343	conflict
Val 3444	Ile 3444	conflict
Arg 3556	Lys 3556	conflict
Asp 3742	Asn 3742	conflict
Val 3895	Phe 3895	conflict
Asp 4072	Asn 4072	conflict

B, C, E, F: 1 - 494 (UniProt: P39946)

PDB	External Database	Details
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	891170.5
Non-Polymers*	Number of molecules	8
	Total formula weight	3897.5
All*	Total formula weight	895068.0

*Water molecules are not included.