8DTI

Cryo-EM structure of Arabidopsis SPY in complex with GDP-fucose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Probable UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase SPINDLY	polymer	946	105278.9	2	UniProt (Q96301) Pfam (PF00515) Pfam (PF13844) In PDB	Arabidopsis thaliana (thale cress)	SPY, GDP-fucose protein O-fucosyltransferase SPINDLY
2	A, B	GUANOSINE-5'-DIPHOSPHATE-BETA-L-FUCOPYRANOSE	non-polymer		589.3	2	Chemie (GFB)

Sequence modifications

A, B: 1 - 914 (UniProt: Q96301)

PDB	External Database	Details
Gly 915	-	expression tag
Gly 916	-	expression tag
Ser 917	-	expression tag
Glu 918	-	expression tag
Asn 919	-	expression tag
Leu 920	-	expression tag
Tyr 921	-	expression tag
Phe 922	-	expression tag
Gln 923	-	expression tag
Gly 924	-	expression tag
Gly 925	-	expression tag
Ser 926	-	expression tag
His 927	-	expression tag
His 928	-	expression tag
His 929	-	expression tag
His 930	-	expression tag
His 931	-	expression tag
His 932	-	expression tag
His 933	-	expression tag
His 934	-	expression tag
His 935	-	expression tag
His 936	-	expression tag
Gly 937	-	expression tag
Gly 938	-	expression tag
Trp 939	-	expression tag
Ser 940	-	expression tag
His 941	-	expression tag
Pro 942	-	expression tag
Gln 943	-	expression tag
Phe 944	-	expression tag
Glu 945	-	expression tag
Lys 946	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	210557.7
Non-Polymers*	Number of molecules	2
	Total formula weight	1178.7
All*	Total formula weight	211736.4

*Water molecules are not included.