8DDX
cryo-EM structure of TRPM3 ion channel in complex with Gbg in the presence of PIP2, tethered by ALFA-nanobody
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, E, G (A, B, C, D) | Transient receptor potential cation channel, subfamily M, member 3 | polymer | 1370 | 157681.7 | 4 | UniProt (Q5F4S7) Pfam (PF18139) Pfam (PF00520) Pfam (PF16519) UniProt (by SIFTS) (J9SQF3) | Mus musculus (house mouse) | |
| 2 | B, D, F, H (E, F, G, H) | Unidentified segment at the N-terminus of TRPM3 | polymer | 17 | 1464.8 | 4 | Mus musculus | ||
| 3 | I (I) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37416.9 | 1 | UniProt (P62873) Pfam (PF00400) | Homo sapiens (human) | Transducin beta chain 1 |
| 4 | J (J) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 70 | 7713.9 | 1 | UniProt (P59768) Pfam (PF00631) | Homo sapiens (human) | G gamma-I |
| 5 | K, O, R, V (A, B, C, D) | 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE | non-polymer | 705.0 | 4 | Chemie (3PH) | |||
| 6 | L, P, T, W (A, B, C, D) | [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate | non-polymer | 746.6 | 4 | Chemie (PIO) | |||
| 7 | M, N, Q, S (A, B, C) | (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en | non-polymer | 544.8 | 4 | Chemie (9Z9) | |||
| 8 | U (C) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) |
Sequence modifications
J: 2 - 71 (UniProt: P59768)
| PDB | External Database | Details |
|---|---|---|
| Ser 68 | Cys 68 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 681716.9 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 8008.5 | |
| All* | Total formula weight | 689725.4 |
