Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

8DDX

cryo-EM structure of TRPM3 ion channel in complex with Gbg in the presence of PIP2, tethered by ALFA-nanobody

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005216	molecular_function	monoatomic ion channel activity
A	0006811	biological_process	monoatomic ion transport
A	0016020	cellular_component	membrane
A	0051262	biological_process	protein tetramerization
A	0055085	biological_process	transmembrane transport
B	0005216	molecular_function	monoatomic ion channel activity
B	0006811	biological_process	monoatomic ion transport
B	0016020	cellular_component	membrane
B	0051262	biological_process	protein tetramerization
B	0055085	biological_process	transmembrane transport
C	0005216	molecular_function	monoatomic ion channel activity
C	0006811	biological_process	monoatomic ion transport
C	0016020	cellular_component	membrane
C	0051262	biological_process	protein tetramerization
C	0055085	biological_process	transmembrane transport
D	0005216	molecular_function	monoatomic ion channel activity
D	0006811	biological_process	monoatomic ion transport
D	0016020	cellular_component	membrane
D	0051262	biological_process	protein tetramerization
D	0055085	biological_process	transmembrane transport
I	0001750	cellular_component	photoreceptor outer segment
I	0003924	molecular_function	GTPase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005765	cellular_component	lysosomal membrane
I	0005829	cellular_component	cytosol
I	0005834	cellular_component	heterotrimeric G-protein complex
I	0005886	cellular_component	plasma membrane
I	0007165	biological_process	signal transduction
I	0007186	biological_process	G protein-coupled receptor signaling pathway
I	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
I	0007200	biological_process	phospholipase C-activating G protein-coupled receptor signaling pathway
I	0007213	biological_process	G protein-coupled acetylcholine receptor signaling pathway
I	0007265	biological_process	Ras protein signal transduction
I	0008283	biological_process	cell population proliferation
I	0016020	cellular_component	membrane
I	0030159	molecular_function	signaling receptor complex adaptor activity
I	0044877	molecular_function	protein-containing complex binding
I	0045202	cellular_component	synapse
I	0050909	biological_process	sensory perception of taste
I	0051020	molecular_function	GTPase binding
I	0060041	biological_process	retina development in camera-type eye
I	0070062	cellular_component	extracellular exosome
I	0071380	biological_process	cellular response to prostaglandin E stimulus
I	0071870	biological_process	cellular response to catecholamine stimulus
I	0097381	cellular_component	photoreceptor disc membrane
I	1903561	cellular_component	extracellular vesicle
J	0005515	molecular_function	protein binding
J	0005834	cellular_component	heterotrimeric G-protein complex
J	0005886	cellular_component	plasma membrane
J	0007165	biological_process	signal transduction
J	0007186	biological_process	G protein-coupled receptor signaling pathway
J	0007191	biological_process	adenylate cyclase-activating dopamine receptor signaling pathway
J	0016020	cellular_component	membrane
J	0031681	molecular_function	G-protein beta-subunit binding
J	0045202	cellular_component	synapse
J	0048144	biological_process	fibroblast proliferation
J	0070062	cellular_component	extracellular exosome
J	0071380	biological_process	cellular response to prostaglandin E stimulus
J	0071870	biological_process	cellular response to catecholamine stimulus

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LVSAsqDgKLIIWDS

Chain	Residue	Details
I	LEU70-SER84
I	ILE157-ILE171
I	LEU285-ALA299

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	92
Details	Transmembrane: {"description":"Helical; Name=1","evidences":[{"source":"PubMed","id":"36283409","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	100
Details	Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	88
Details	Transmembrane: {"description":"Helical; Name=2","evidences":[{"source":"PubMed","id":"36283409","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	116
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	80
Details	Transmembrane: {"description":"Helical; Name=3","evidences":[{"source":"PubMed","id":"36283409","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	92
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"PubMed","id":"36283409","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	108
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"PubMed","id":"36283409","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	100
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"PubMed","id":"36283409","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	299
Details	Region: {"description":"Calmodulin-binding","evidences":[{"source":"PubMed","id":"29880196","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36283409","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8DDU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"PubMed","id":"36283409","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8DDT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8DDU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	8
Details	Region: {"description":"Required for the inhibitory action of G-beta/gamma-subunits of heterotrimeric G-proteins","evidences":[{"source":"PubMed","id":"33122432","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	48
Details	Repeat: {"description":"WD 1","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	45
Details	Repeat: {"description":"WD 2","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	40
Details	Repeat: {"description":"WD 3","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	41
Details	Repeat: {"description":"WD 4","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	43
Details	Repeat: {"description":"WD 5","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	41
Details	Repeat: {"description":"WD 6","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	30
Details	Repeat: {"description":"WD 7","evidences":[{"source":"PubMed","id":"38600377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"38776963","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"8VY7","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8VY9","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQN","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQR","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQS","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8XQT","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8YKY","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	1
Details	Modified residue: {"description":"Phosphohistidine","evidences":[{"source":"UniProtKB","id":"P62871","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	1
Details	Modified residue: {"description":"Cysteine methyl ester","evidences":[{"source":"UniProtKB","id":"P63212","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	1
Details	Lipidation: {"description":"S-geranylgeranyl cysteine","evidences":[{"source":"UniProtKB","id":"P63212","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)