8D74

Cryo-EM structure of human CNTF signaling complex: model containing the interaction core region

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Interleukin-6 receptor subunit beta	polymer	625	71233.2	1	UniProt (P40189) Pfam (PF06328) Pfam (PF09240) Pfam (PF00041)	Homo sapiens (human)	IL-6 receptor subunit beta,IL-6R subunit beta,IL-6R-beta,IL-6RB,CDw130,Interleukin-6 signal transducer,Membrane glycoprotein 130,gp130,Oncostatin-M receptor subunit alpha
2	B (D)	Ciliary neurotrophic factor	polymer	214	24704.9	1	UniProt (P26441) Pfam (PF01110)	Homo sapiens (human)	CNTF
3	C (C)	Ciliary neurotrophic factor receptor subunit alpha	polymer	320	35855.3	1	UniProt (P26992) Pfam (PF00041)	Homo sapiens (human)	CNTF receptor subunit alpha,CNTFR-alpha
4	D (B)	Leukemia inhibitory factor receptor	polymer	817	92834.8	1	UniProt (P42702) Pfam (PF18207) Pfam (PF17971) Pfam (PF21177) Pfam (PF00041)	Homo sapiens (human)	LIF receptor,LIF-R
5	E, F, G, H, I... (E, F, G, H, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	6	In PDB GlyTouCan (G42666HT)
6	K, L, M, N, O... (A, C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A: 23 - 619 (UniProt: P40189)

PDB	External Database	Details
Glu 620	-	expression tag
Gln 621	-	expression tag
Lys 622	-	expression tag
Leu 623	-	expression tag
Ile 624	-	expression tag
Ser 625	-	expression tag
Glu 626	-	expression tag
Glu 627	-	expression tag
Asp 628	-	expression tag
Leu 629	-	expression tag
Gly 630	-	expression tag
Gly 631	-	expression tag
Glu 632	-	expression tag
Gln 633	-	expression tag
Lys 634	-	expression tag
Leu 635	-	expression tag
Ile 636	-	expression tag
Ser 637	-	expression tag
Glu 638	-	expression tag
Glu 639	-	expression tag
Asp 640	-	expression tag
Leu 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag
His 646	-	expression tag
His 647	-	expression tag

D: 1 - 186 (UniProt: P26441)

PDB	External Database	Details
Glu 187	-	expression tag
Gln 188	-	expression tag
Lys 189	-	expression tag
Leu 190	-	expression tag
Ile 191	-	expression tag
Ser 192	-	expression tag
Glu 193	-	expression tag
Glu 194	-	expression tag
Asp 195	-	expression tag
Leu 196	-	expression tag
Gly 197	-	expression tag
Gly 198	-	expression tag
Glu 199	-	expression tag
Gln 200	-	expression tag
Lys 201	-	expression tag
Leu 202	-	expression tag
Ile 203	-	expression tag
Ser 204	-	expression tag
Glu 205	-	expression tag
Glu 206	-	expression tag
Asp 207	-	expression tag
Leu 208	-	expression tag
His 209	-	expression tag
His 210	-	expression tag
His 211	-	expression tag
His 212	-	expression tag
His 213	-	expression tag
His 214	-	expression tag

B: 45 - 833 (UniProt: P42702)

PDB	External Database	Details
Glu 834	-	expression tag
Gln 835	-	expression tag
Lys 836	-	expression tag
Leu 837	-	expression tag
Ile 838	-	expression tag
Ser 839	-	expression tag
Glu 840	-	expression tag
Glu 841	-	expression tag
Asp 842	-	expression tag
Leu 843	-	expression tag
Gly 844	-	expression tag
Gly 845	-	expression tag
Glu 846	-	expression tag
Gln 847	-	expression tag
Lys 848	-	expression tag
Leu 849	-	expression tag
Ile 850	-	expression tag
Ser 851	-	expression tag
Glu 852	-	expression tag
Glu 853	-	expression tag
Asp 854	-	expression tag
Leu 855	-	expression tag
His 856	-	expression tag
His 857	-	expression tag
His 858	-	expression tag
His 859	-	expression tag
His 860	-	expression tag
His 861	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	224628.2
Branched	Number of molecules	6
	Total formula weight	2546.4
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	228501.8

*Water molecules are not included.