8D58

Crystal structure of human METTL1-WDR4 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	tRNA (guanine-N(7)-)-methyltransferase	polymer	246	28456.6	1	UniProt (Q9UBP6) Pfam (PF02390)	Homo sapiens (human)	Methyltransferase-like protein 1,mRNA (guanine-N(7)-)-methyltransferase,miRNA (guanine-N(7)-)-methyltransferase,tRNA (guanine(46)-N(7))-methyltransferase,tRNA(m7G46)-methyltransferase
2	B (B)	tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4	polymer	405	45123.0	1	UniProt (P57081) Pfam (PF00400)	Homo sapiens (human)	Protein Wuho homolog,hWH,WD repeat-containing protein 4
3	C, D, G, H, I... (A, B)	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
4	E, K (A, B)	ISOPROPYL ALCOHOL	non-polymer		60.1	2	Chemie (IPA)
5	F (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
6	L, M (A, B)	water	water		18.0	60	Chemie (HOH)

Sequence modifications

B: 1 - 389 (UniProt: P57081)

PDB	External Database	Details
Met -15	-	initiating methionine
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	73579.6
Non-Polymers*	Number of molecules	9
	Total formula weight	425.0
All*	Total formula weight	74004.6

*Water molecules are not included.