8D0K

Human CST-DNA polymerase alpha/primase preinitiation complex bound to 4xTEL-foldback template - PRIM2C advanced PIC

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CST complex subunit CTC1	polymer	1246	138030.3	1	UniProt (Q2NKJ3) Pfam (PF15489)	Homo sapiens (human)
2	B (B)	CST complex subunit STN1	polymer	374	43001.8	1	UniProt (Q9H668) Pfam (PF01336) Pfam (PF09170)	Homo sapiens (human)	Oligonucleotide/oligosaccharide-binding fold-containing protein 1,Suppressor of cdc thirteen homolog
3	C (C)	CST complex subunit TEN1	polymer	152	17285.6	1	UniProt (Q86WV5) Pfam (PF15490)	Homo sapiens (human)	Protein telomeric pathways with STN1 homolog,Telomere length regulation protein TEN1 homolog
4	D (D)	DNA primase small subunit	polymer	434	51356.4	1	UniProt (P49642) Pfam (PF01896)	Homo sapiens (human)	DNA primase 49 kDa subunit,p49
5	E (E)	DNA primase large subunit	polymer	523	60266.3	1	UniProt (P49643) Pfam (PF04104)	Homo sapiens (human)	DNA primase 58 kDa subunit,p58
6	F (F)	DNA polymerase alpha catalytic subunit	polymer	1527	172796.9	1	UniProt (P09884) Pfam (PF08996) Pfam (PF12254) Pfam (PF03104) Pfam (PF00136)	Homo sapiens (human)	DNA polymerase alpha catalytic subunit p180
7	G (G)	DNA polymerase alpha subunit B	polymer	612	67390.9	1	UniProt (Q14181) Pfam (PF08418) Pfam (PF22062) Pfam (PF04042)	Homo sapiens (human)	DNA polymerase alpha 70 kDa subunit
8	H (H)	DNA (5'-D(P*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*TP*TP*A)-3')	polymer	60	18670.9	1	GenBank (1894914083)	Homo sapiens

Sequence modifications

A: 2 - 1217 (UniProt: Q2NKJ3)

PDB	External Database	Details
Met -28	-	initiating methionine
Gly -27	-	expression tag
Asp -26	-	expression tag
Tyr -25	-	expression tag
Lys -24	-	expression tag
Asp -23	-	expression tag
His -22	-	expression tag
Asp -21	-	expression tag
Gly -20	-	expression tag
Asp -19	-	expression tag
Tyr -18	-	expression tag
Lys -17	-	expression tag
Asp -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Ile -13	-	expression tag
Asp -12	-	expression tag
Tyr -11	-	expression tag
Lys -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Lys -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Leu 0	-	expression tag
Glu 1	-	expression tag
Val 820	Ile 820	conflict
Val 1005	Ile 1005	conflict

B: 2 - 368 (UniProt: Q9H668)

PDB	External Database	Details
Met -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag

C: 3 - 123 (UniProt: Q86WV5)

PDB	External Database	Details
Met -28	-	expression tag
Ser -27	-	expression tag
Tyr -26	-	expression tag
Tyr -25	-	expression tag
His -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
Asp -18	-	expression tag
Tyr -17	-	expression tag
Asp -16	-	expression tag
Ile -15	-	expression tag
Pro -14	-	expression tag
Thr -13	-	expression tag
Thr -12	-	expression tag
Glu -11	-	expression tag
Asn -10	-	expression tag
Leu -9	-	expression tag
Tyr -8	-	expression tag
Phe -7	-	expression tag
Gln -6	-	expression tag
Gly -5	-	expression tag
Ala -4	-	expression tag
Met -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Ile 1	-	expression tag
Gln 2	-	expression tag

D: 2 - 420 (UniProt: P49642)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

E: 2 - 509 (UniProt: P49643)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

F: 2 - 1462 (UniProt: P09884)

PDB	External Database	Details
Met -63	-	initiating methionine
Gly -62	-	expression tag
Gly -61	-	expression tag
Ser -60	-	expression tag
Ala -59	-	expression tag
Gly -58	-	expression tag
Asp -57	-	expression tag
Tyr -56	-	expression tag
Lys -55	-	expression tag
Asp -54	-	expression tag
His -53	-	expression tag
Asp -52	-	expression tag
Gly -51	-	expression tag
Asp -50	-	expression tag
Tyr -49	-	expression tag
Lys -48	-	expression tag
Asp -47	-	expression tag
His -46	-	expression tag
Asp -45	-	expression tag
Ile -44	-	expression tag
Asp -43	-	expression tag
Tyr -42	-	expression tag
Lys -41	-	expression tag
Asp -40	-	expression tag
Asp -39	-	expression tag
Asp -38	-	expression tag
Asp -37	-	expression tag
Lys -36	-	expression tag
Gly -35	-	expression tag
Ala -34	-	expression tag
Ser -33	-	expression tag
Ser -32	-	expression tag
Ala -31	-	expression tag
Trp -30	-	expression tag
Ser -29	-	expression tag
His -28	-	expression tag
Pro -27	-	expression tag
Gln -26	-	expression tag
Phe -25	-	expression tag
Glu -24	-	expression tag
Lys -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Gly -18	-	expression tag
Gly -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Gly -13	-	expression tag
Ser -12	-	expression tag
Ala -11	-	expression tag
Trp -10	-	expression tag
Ser -9	-	expression tag
His -8	-	expression tag
Pro -7	-	expression tag
Gln -6	-	expression tag
Phe -5	-	expression tag
Glu -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Val 1463	-	expression tag

G: 2 - 598 (UniProt: Q14181)

PDB	External Database	Details
Met -13	-	initiating methionine
Gly -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

H: -7 - 23 (GenBank: 1894914083)

PDB	External Database	Details
Dc -36	-	expression tag
Dt -35	-	expression tag
Da -34	-	expression tag
Da -33	-	expression tag
Dc -32	-	expression tag
Dc -31	-	expression tag
Dg -30	-	expression tag
Dc -29	-	expression tag
Da -28	-	expression tag
Dt -27	-	expression tag
Dc -26	-	expression tag
Dt -25	-	expression tag
Da -24	-	expression tag
Dg -23	-	expression tag
Dc -22	-	expression tag
Dt -21	-	expression tag
Dt -20	-	expression tag
Dt -19	-	expression tag
Dt -18	-	expression tag
Dt -17	-	expression tag
Dg -16	-	expression tag
Dc -15	-	expression tag
Dt -14	-	expression tag
Da -13	-	expression tag
Dg -12	-	expression tag
Da -11	-	expression tag
Dt -10	-	expression tag
Dg -9	-	expression tag
Dc -8	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	568799.0
All*	Total formula weight	568799.0

*Water molecules are not included.