8CTH
Cryo-EM structure of human METTL1-WDR4-tRNA(Phe) complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | tRNA (guanine-N(7)-)-methyltransferase | polymer | 276 | 31516.0 | 1 | UniProt (Q9UBP6) Pfam (PF02390) | Homo sapiens (human) | Methyltransferase-like protein 1,mRNA (guanine-N(7)-)-methyltransferase,miRNA (guanine-N(7)-)-methyltransferase,tRNA (guanine(46)-N(7))-methyltransferase,tRNA(m7G46)-methyltransferase |
| 2 | B (B) | tRNA (guanine-N(7)-)-methyltransferase non-catalytic subunit WDR4 | polymer | 428 | 47363.5 | 1 | UniProt (P57081) Pfam (PF00400) | Homo sapiens (human) | Protein Wuho homolog,hWH,WD repeat-containing protein 4 |
| 3 | C (C) | Phe-tRNA | polymer | 76 | 24650.9 | 1 | Saccharomyces cerevisiae (Yeast) | ||
| 4 | D (A) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) |
Sequence modifications
B: 1 - 412 (UniProt: P57081)
| PDB | External Database | Details |
|---|---|---|
| Met -15 | - | initiating methionine |
| Gly -14 | - | expression tag |
| Ser -13 | - | expression tag |
| Ser -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| Ser -5 | - | expression tag |
| Gln -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 103530.4 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 384.4 | |
| All* | Total formula weight | 103914.8 |
