8CTG

Extracellular architecture of an engineered canonical Wnt signaling ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Frizzled-8	polymer	132	15061.3	1	UniProt (Q61091) Pfam (PF01392)	Mus musculus (house mouse)	Fz-8,mFz8
2	B (B)	Protein Wnt-8	polymer	327	36676.6	1	UniProt (P28026) Pfam (PF00110)	Xenopus laevis (African clawed frog)	XWnt-8
3	C (C)	Low-density lipoprotein receptor-related protein 6	polymer	649	73384.6	1	UniProt (O75581) Pfam (PF14670) Pfam (PF00058)	Homo sapiens (human)	LRP-6
4	D (B)	PALMITOLEIC ACID	non-polymer		254.4	1	Chemie (PAM)

Sequence modifications

A: 28 - 150 (UniProt: Q61091)

PDB	External Database	Details
Ala 151	-	expression tag
Ala 152	-	expression tag
Ala 153	-	expression tag
Leu 154	-	expression tag
Glu 155	-	expression tag
Val 156	-	expression tag
Leu 157	-	expression tag
Phe 158	-	expression tag
Gln 159	-	expression tag

B: 22 - 329 (UniProt: P28026)

PDB	External Database	Details
Gly 21	-	expression tag
Pro 227	Arg 227	conflict
Thr 229	Gly 229	conflict
Val 232	Ala 232	conflict
Asn 233	Asp 233	conflict
Ser 234	Asn 234	conflict
Arg 236	Gly 236	conflict
Gln 330	-	expression tag
Val 331	-	expression tag
Val 332	-	expression tag
Ile 333	-	expression tag
Lys 334	-	expression tag
His 335	-	expression tag
Phe 336	-	expression tag
Cys 337	-	expression tag
Ala 338	-	expression tag
Arg 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag

C: 20 - 629 (UniProt: O75581)

PDB	External Database	Details
Gly 18	-	expression tag
Ser 19	-	expression tag
Ser 630	-	expression tag
Arg 631	-	expression tag
Gly 632	-	expression tag
Leu 633	-	expression tag
Glu 634	-	expression tag
Val 635	-	expression tag
Leu 636	-	expression tag
Phe 637	-	expression tag
Gln 638	-	expression tag
Gly 639	-	expression tag
Pro 640	-	expression tag
Gly 641	-	expression tag
Ala 642	-	expression tag
Ala 643	-	expression tag
Gly 644	-	expression tag
Leu 645	-	expression tag
Asn 646	-	expression tag
Asp 647	-	expression tag
Ile 648	-	expression tag
Phe 649	-	expression tag
Glu 650	-	expression tag
Ala 651	-	expression tag
Gln 652	-	expression tag
Lys 653	-	expression tag
Ile 654	-	expression tag
Glu 655	-	expression tag
Trp 656	-	expression tag
His 657	-	expression tag
Glu 658	-	expression tag
His 659	-	expression tag
His 660	-	expression tag
His 661	-	expression tag
His 662	-	expression tag
His 663	-	expression tag
His 664	-	expression tag
His 665	-	expression tag
His 666	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	125122.6
Non-Polymers*	Number of molecules	1
	Total formula weight	254.4
All*	Total formula weight	125377.0

*Water molecules are not included.