8CR3

Crystal structure of recombinant LasB from Pseudomonas aeruginosa PAO1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Pro-elastase	polymer	514	55672.9	1	UniProt (P14756) Pfam (PF07504) Pfam (PF03413) Pfam (PF01447) Pfam (PF02868) In PDB	Pseudomonas aeruginosa PAO1
2	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
3	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
4		water	water		18.0	410	Chemie (HOH)

Sequence modifications

A: -173 - 301 (UniProt: P14756)

PDB	External Database	Details
Met -197	-	initiating methionine
Lys -196	-	expression tag
Tyr -195	-	expression tag
Leu -194	-	expression tag
Leu -193	-	expression tag
Pro -192	-	expression tag
Thr -191	-	expression tag
Ala -190	-	expression tag
Ala -189	-	expression tag
Ala -188	-	expression tag
Gly -187	-	expression tag
Leu -186	-	expression tag
Leu -185	-	expression tag
Leu -184	-	expression tag
Leu -183	-	expression tag
Ala -182	-	expression tag
Ala -181	-	expression tag
Gln -180	-	expression tag
Pro -179	-	expression tag
Ala -178	-	expression tag
Met -177	-	expression tag
Ala -176	-	expression tag
Met -175	-	expression tag
Gly -174	-	expression tag
Glu 302	-	expression tag
Asn 303	-	expression tag
Leu 304	-	expression tag
Tyr 305	-	expression tag
Phe 306	-	expression tag
Gln 307	-	expression tag
Gly 308	-	expression tag
Leu 309	-	expression tag
Glu 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55672.9
Non-Polymers*	Number of molecules	2
	Total formula weight	105.5
All*	Total formula weight	55778.4

*Water molecules are not included.